An artificial amyloid-based redox hydrogel was designed for mediating electron transfer between a [NiFeSe] hydrogenase and an electrode. Starting from mutated prion-forming domain of fungal protein HET-s, hybrid containing single benzyl methyl viologen moiety synthesized. This able to self-assemble into structurally homogenous nanofibrils. Molecular modeling confirmed that the groups are aligne...

Cyclic peptides are increasingly being shown as powerful inhibitors of fibril formation, and have the potential to be therapeutic agents for combating many debilitating amyloid-related diseases. One such example is a cyclic peptide derivative from the human apolipoprotein C-II, which has the ability to inhibit fibril formation by the fibrillogenic peptide apoC-II(60-70). Using classical molecul...

Aggregation of α-synuclein (αSyn), the primary protein component in Lewy body inclusions of patients with Parkinson's disease, arises when the normally soluble intrinsically disordered protein converts to amyloid fibrils. In this work, we provide a mechanistic view of the role of N-terminal acetylation on fibrillation by first establishing a quantitative relationship between monomer secondary s...

Fibril formation resulting from protein misfolding and aggregation is a hallmark of several neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Despite the fact that the fibril formation process is very slow and thus poses a significant challenge for theoretical and experimental studies, a number of alternative pictures of molecular mechanisms of amyloid fibril formation ha...

The unique enhanced sensitivity of vibrational circular dichroism (VCD) to the formation and development of amyloid fibrils in solution is extended to four additional fibril-forming proteins or peptides where it is shown that the sign of the fibril VCD pattern correlates with the sense of supramolecular filament chirality and, without exception, to the dominant fibril morphology as observed in ...

This study investigated for the first time the molecular effectiveness of 'aroma' from three small molecules including a phenol (phenyl ethyl alcohol; PEA) and an aldehyde (cinnamaldehyde; Cin) both containing an aromatic ring, and a diamine (N,N,N,N'- Tetramethylethylenediamine; TEMED) at two different amounts (small; S and large; L) in preventing hen egg white lysozyme (HEWL) amyloid fibril f...

Understanding the damage and failure of load-carrying soft biological tissue is critical in effectual treatment injury disease. The difficulty experimentally identifying intrinsic mechanisms by which initiates accumulates, how this ultimately leads to rupture, has motivated constitutive modeling failure. We present an extension our previous microstructural continuum model (Miller Gasser, 2021) ...

Soluble rat tail tendon collagen with intact nonhelical ends and largely free of aggregates was used to study fibril formation in vitro. The process was initiated by raising the pH and warming a cold solution of collagen. Fibril formation was monitored by turbidity and the product was examined by electron microscopy. Optimal conditions that will form fibrils similar to those observed in vivo ar...

Extracellular matrix (ECM) can be considered as a biological example of a fibre-reinforced composite material in which collagen fibrils provide tensile reinforcement (Hukins and Aspden, 1985). The fibrils are surrounded by a weak proteoglycan (PG) gel and may have other molecules, such as decorin and biglycan, closely associated with them (Hedbom and Heinegard, 1993; Roughley and Lee, 1994). Si...

An artificial amyloid-based redox hydrogel was designed for mediating electron transfer between a [NiFeSe] hydrogenase and an electrode. Starting from mutated prion-forming domain of fungal protein HET-s, hybrid containing single benzyl methyl viologen moiety synthesized. This able to self-assemble into structurally homogenous nanofibrils. Molecular modeling confirmed that the groups are aligne...