BACKGROUND Aberrant promoter methylation is a major mechanism for silencing tumor suppressor genes in cancer. Detection of hypermethylation is used as a molecular marker for early cancer diagnosis, as a prognostic index, or to define therapeutic targets for reversion of aberrant methylation. We report on a novel signal generation technology for real-time PCR to detect gene promoter methylation....

Background: Aberrant promoter methylation is a major mechanism for silencing tumor suppressor genes in cancer. Detection of hypermethylation is used as a molecular marker for early cancer diagnosis, as a prognostic index, or to define therapeutic targets for reversion of aberrant methylation. We report on a novel signal generation technology for real-time PCR to detect gene promoter methylation...

epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a  chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...

The Mailbox Problem was described and solved by Aguilera, Gafni, and Lamport in [4] with an algorithm that uses two flag registers that carry 14 values each. An interesting problem that they ask is whether there is a mailbox algorithm with smaller flag values. We give a positive answer by describing a mailbox algorithm with 6 and 4 values in the two flag registers.

In the present study, we investigated the accuracy of offside judgements of assistant referees in the English Premier League. The moment in the match, the position and movement speed of the assistant referee, attacker and second-last defender, together with the angle of view for the assistant referee were all considered to underlie incorrect decisions. The error rate was 17.5% (868 of 4960 situ...

The human cytomegalovirus (HCMV)-encoded viral G protein-coupled receptor pUS28 contributes to an array of biological effects, including cell migration and proliferation. Using FIX-BAC (bacterial artificial chromosome, derived from the HCMV clinical isolate VR1814) and lambda red recombination techniques, we generated HCMV recombinants expressing amino-terminally FLAG-tagged versions of wild-ty...

Objective. Existing research suggests that conservative racial attitudes are one of the strongest factors explaining support for the Confederate flag, but this conclusion has been reached by examining the attitudes of only white southerners. We provide a more complete understanding of this issue, focusing on both white and black opinion from across the country. Methods. We use a rolling cross-s...