The effects of native cyclodextrins (alpha, beta or gamma), hydroxypropyl-beta-cyclodextrin, beta-cyclodextrin solubilized in urea, soluble starch and glucose in water solution on the fluorescence behaviour of melatonin (N-acetyl-5-methoxytryptamine) (M) and 5-methoxytryptamine [5-methoxy-3-(2-aminoethyl)indole] (5M) were determined. In addition, the effect of methanol and propanol with and wit...

BACKGROUND Circulating autoantibodies to pancreatic glutamic acid decarboxylase (GAD65; the 65-kDa isoform of glutamic acid decarboxylase), protein tyrosine phosphatase-like protein IA-2, and insulin can be used as predictive markers of type 1 diabetes. We developed a novel assay for the detection of IA-2 autoantibodies (IA-2As) in serum based on time-resolved fluorimetry, hypothesizing that th...

This work describes the development of flow analysis microsystems with integrated fluorimetric detection cells. Channels (width of 300-540 microm and depth of 200-590 microm) were manufactured by deep-UV lithography in urethane-acrylate (UA) resin. Plastic optical fibers (diameter of 250 microm) were coupled to a 2.0-mm-long detection channel in order to guide the excitation radiation from an L...

Comparison of fluorescence intensities of corticosterone and cortisol in a variety of concentrations of sulphuric acid to ethanol has led to the finding that corticosterone gives several times as high fluorescence as cortisol in reagent consist ing of 6:4(v/v) sulphuric acid and ethanol, while it produces low fluorescence in comparison with cortisol in 9:1(v/v) sulphuric acid-ethanol reagent. T...

Differences in color of the skin and hair of animals have been the subject of much research. Such differences have been ascribed partly to differences in the relative amounts of dark eumelanins, and lighter pigments, phaeomelanins, in the pigmented tissues. The chemistry of eumelanins has been extensively discussed in the classic monograph of Nicolaus (1). The early work of Prota and Nicolaus s...

A simple spectrofluorimetric analysis of a local anaesthetic named procaine using a specific labelling reagent for primary amino groups, has been developed. Because procaine shows very weak native fluorescence, the technique of spectrofluorimetry has been very much limited for its determination. A detail study of the variables affecting the derivatisation reaction (pH, fluorescamine (FC) concen...

Обсуждается применение флуориметрического метода анализа в экологическом мониторинге рек г. Твери и Иваньковского водохранилища. Приведены результаты содержания нефтепродуктов ряде водных объектов Тверской области. The application of the fluorimetric method analysis in ecological monitoring rivers city Tver and Ivankovo reservoir is discussed. results content oil products a number water bodies ...