A bis-β-diketone, bis(4,4,4-trifluoro-1,3-dioxobutyl)(2,2'-bithienyl) (BTT), which can be looked upon as coupling of two mono-β-diketones (2-thenoyltrifluoroacetone, TTA) at the 5,5'-position of thiophene ring, has been designed for exploring the advantages of binuclear helical structure in sensitizing the lanthanide NIR luminescence. The Yb(iii) ion was selected as the luminescent center, and ...

We performed density functional theory (DFT) calculations on binary and ternary oxo/fluoro crystals of the geochemical twin pair zirconium hafnium to evaluate compare their stabilities. This is first DFT study bulk ZrF4 or HfF4, as well a hypothetical ZrOF2 HfOF2 crystal. For α-MO2, β-MF4 MOF2, we have found significantly higher cohesive energies for respective species. suggests considerable ga...

Surface plasmon resonance (SPR) was used to measure polymerase-binding interactions of the bulky mutagenic DNA lesions N-(2'-deoxyguanosin-8-yl)-4'-fluoro-4-aminobiphenyl (FABP) or N-(2'-deoxyguanosin-8-yl)-7-fluoro-2-acetylaminofluorene (FAAF) in the context of two unique 5'-flanking bases (CG*A and TG*A). The enzymes used were exo-nuclease-deficient Klenow fragment (Kf-exo(-)) or polymerase β...

Aldolase antibodies that operate via an enamine mechanism were developed by in vitro selection. Antibody Fab phage display libraries were created where the catalytic active site residues of aldolase antibodies 38C2 and 33F12 were combined with a naive human antibody V gene repertoire. Selection from these libraries with 1,3-diketones covalently trapped the amino groups of reactive lysine residu...