Cdr1p is the major ATP-binding cassette multidrug transporter conferring resistance to azoles and other antifungals in Candida albicans. In this study, the identification of new Cdr1p inhibitors by use of a newly developed high-throughput fluorescence-based assay is reported. The assay also allowed monitoring of the activity and inhibition of the related transporters Pdr5p and Snq2p of Saccharo...

Dr. James K. Onwubalili, MRC Research Fellow and Senior Registrar, Division of Communicable Diseases, Clinical Research Centre and Northwick Park Hospital, Watford Road, Harrow, Middx, HA1 3UJ (England) Sir, Benoxaprofen was withdrawn in Britain in August 1982 following reports of over 3,500 adverse reactions including 61 deaths accumulated since its introduction in 1980. Fatal cases of cholest...

The Malignant Neuroleptic Syndrome (M. N. S.) as a relatively uncommon but potentially lethal complication of neuro-leptic drug treatment was first described in the French literature by Delay el al. (1960), and several cases of so called 'Malig-nant Hyperthermia' in relation to neuro-leptic drug intake were described. It is only in the last few years that this syndrome has received some attenti...

A high-performance liquid chromatographic method has been developed for the simultaneous analysis of the 12 phenothiazines (chlorpromazine, fluphenazine, levomepromazine, perazine, perphenazine, prochlorperazine, profenamine, promethazine, propericiazine, thioproperazine, thioridazine and trifluoperazine) in human serum using HPLC/UV. The separation was achieved using a C(18) reversed-phase col...

The motility characteristics and the behaviour of caput spermatozoa were investigated. After dilution in B2 medium the majority of spermatozoa presented head-to-head agglutination and a twisted flagellum. Only a small population of these spermatozoa developed an anarchic motility pattern. The addition of crude bovine epididymal fluid forward motility protein (FMP) to the incubation medium suppr...

An accurate and precise RP-HPLC method was developed and validated for the determination of carbamazepine and its impurities iminostilbene and iminodibenzyl in a tablet formulation with fluphenazine as an internal standard. Buffer-methanol (50 + 50, v/v) was used as the mobile phase. During validation, specificity, linearity, precision, accuracy, LOD, LOQ, and robustness of the method were test...