Objective: The aim of this study is to establish a method by single nucleotide polymorphism detection from a single cell using the whole genomic amplification and restriction fragment length polymorphism-PCR (RFLPPCR). Methods: Genomic DNA was first prepared and wholly amplified from 80 biopsied blastomeres using the Sure Plex DNA Amplification System. Then, PCR was carried out on a single blas...

THO Complex 5 (THOC5) gene involves in lipid and fatty acid metabolism. The aim of this study was to analyse polymorphism THOC5 its association with composition sheep. A total 120 rams at the age 12 month average body weight 25–30 kg used for identification using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP). 83 rams, representative from different genotypes shee...

Infection by the hepatitis C virus (HCV) is a big threat to human health all over world. Many host factors could affect treatment response in HCV infection. One of these IL28B gene polymorphism. This research study investigated association (rs8099917) polymorphism with patients chronic infection receiving combination Sovaldi, Daklinza, and Ribavirin Egypt. The included 100 patients, divided int...

OBJECTIVES Necrosis of femoral head is a severe pathological state with multiple etiologies. This study investigated the association of the 27-bp repeat polymorphism in intron 4 and G894T polymorphism in exon 7 of the endothelial nitric oxide synthase (eNOS) gene with the pathogenesis of avascular necrosis of femoral head (ANFH). METHODS A total of 125 non-traumatic ANFH patients and 126 heal...

The primary aim of this study was to investigate the status of RANKL6-7 gene polymorphism in patients with chronic (mild, moderate, severe) and aggressive periodontitis as well as healthy controls. We examined80 patients for the RANKL6-7 polymorphisms (rs1054016 and rs9567000). Polymorphism was determinedby polymerase chain reaction (PCR) followed by direct sequencing. No statistica...

Background and Purpose: The epidemiological alteration in the distribution of Candida species, as well as the significantly increasing trend of either intrinsic or acquired resistance of some of these fungi highlights the need for a reliable method for the identification of the species. Polymerase chain reaction (PCR) is one of the methods facilitating the quick and precise identification of Ca...

Abstract Background Migraine is a neurovascular disorder; several studies have demonstrated the immune system plays key role in migraine pathogenesis . The aim of this study was to investigate association between FOXP3 gene polymorphism and susceptibility migraine. Methods In case–control study, 55 whole blood samples patients with 80 healthy were collected. After DNA extraction, genotyping rs5...

Background: TP53 is a recently be suspected to be involves in neural apoptotic events. Also, TP53 codon72 and long-form variants of DRD4 uVNTR polymorphism are reported to be conferred susceptibility to schizophrenia, respectively. Methods: Nine hundred and thirty-four schizophrenia patients and 433 healthy individuals were recruited, genotyped for TP53 codon72 and DRD4 uVNTR polymorphism by us...

Background: Trichostrongylus is an intestinal parasite that highly prevalent in humans and livestock worldwide. There limited information about the prevalence epidemiology of species among infected Mazandaran Province, northern Iran. This study aimed to identify spp. small ruminants using morphometric molecular methods. Materials Methods: Small organs sheep goats, slaughtered were examined for ...