The tumor suppressor p53 binding protein 1 (53BP1) binds to the DNA-binding domain of p53 and enhances p53-mediated transcriptional activation. 53BP1 contains two breast cancer susceptibility gene 1 COOH terminus (BRCT) motifs, which are present in several proteins involved in DNA repair and/or DNA damage-signaling pathways. Thus, we investigated the potential role of 53BP1 in DNA damage-signal...

5-azacytidine and 5-aza-2'-deoxycytidine are clinically used to treat patients with blood neoplasia. Their antileukemic property is mediated by the trapping and the subsequent degradation of a family of proteins, the DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) leading to DNA demethylation, tumor suppressor gene re-expression and DNA damage. Here we studied the respective role of each DNM...

Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. The mice were pretreated orally with different doses of (50, 100, 20...

Objective: To evaluate low-molecular-weight (LMW) DNA as possible prognostic biomarker in acute ischemic and hemorrhagic stroke. Methods:  LMW DNA samples were isolated from plasma and cerebrospinal fluid by phenol deproteinization, analyzed by gradient polyacrylamide electrophoresis and quantified by spectrophotometry. Results: Two common types of the stroke, ischemic and hemorrhagic, differ...

Damage to cell and tissues by oxidation of polyunsaturated fatty acids (PUFA) is one of the most important risk factors of many diseases such as atherosclerosis, roumatoid arthritis and cancer. Oxidation occurs due to increase of oxidant agents such as free radicals or decrease of antioxidants. Thymus daenensis Celak. is a herbaceous wild medicinal plant endemic to Iran. In the course of screen...

Müller glia are capable of de-differentiating and proliferating to become Müller glia-derived progenitor cells (MGPCs) with the ability to regenerate retinal neurons. One of the cell-signaling pathways that drives the reprogramming of Müller glia into MGPCs in the zebrafish retina is the Jak/Stat-pathway. However, nothing is known about the influence of Jak/Stat-signaling during the formation o...

in this paper, the capabilities of displacement curvature derived from static response data for finding the location and severity of damage in euler-bernoulli beams are assessed. static response of a beam is obtained using the finite element modeling. in order to reduce the number of measured nodal displacements, the beam deflection is fitted through a polynomial function using a limited number...

Age-dependent structural changes in brainstem reticular formation were studied. The significant decrease of mean number of neurons in reticular formation was revealed with age increase. The loss of neurons at maximal level was revealed in patients aged 70 years or over. The loss of neurons may result in constant significant damage to functions of reticular formation and may underlie the dysregu...

Hypertensive patients exhibit elevated cancer incidence, especially of cancers of the kidney. Elevated levels of ANG II, the active peptide of the renin-angiotensin system, regulating blood pressure and cardiovascular homeostasis, are known to cause hypertension and kidney diseases. There is evidence that ANG II is an activator of NAD(P)H oxidase, leading to the formation of free radicals, whic...