Treatment with proinflammatory prostaglandin E2 (PGE2) produced a transient sensitization of whole-cell currents elicited by the vanilloid capsaicin. The intracellular signaling pathways that mediate the initiation of this PGE2-induced sensitization of the capsaicin-elicited current in rat sensory neurons are not well established. Treatment with either forskolin (100 nM to 10 microM) or membran...

In the present study, Western-blot and radioreceptor analyses have revealed the presence of the epidermal growth factor (EGF) receptor in pancreatic acinar membranes. Isolated pancreatic acinar membranes, which allow access of functional antibodies to individual components of the signal transduction cascade, were used to examine EGF-induced regulation of adenylate cyclase activity. Forskolin, v...

Urea transport, mediated by the urea transporter A1 (UT-A1) and/or UT-A3, is important for the production of concentrated urine. Vasopressin rapidly increases urea transport in rat terminal inner medullary collecting ducts (IMCD). A previous study showed that one mechanism for rapid regulation of urea transport is a vasopressin-induced increase in UT-A1 phosphorylation. This study tests whether...

We analyzed whether the cyclic AMP induced down-regulation of the nitric oxide (NO) receptor soluble guanylyl cyclase (sGC) is mediated by the mRNA-protecting protein HuR. Exposure (up to 24 h) of isolated rat aortic segments to the activator of adenylyl cyclase, forskolin (10 microM), and to both activators of cAMP-stimulated protein kinase (PKA), 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazo...

We have recently reported that interleukin-1alpha (IL-1alpha) can induce human macrophage colony-stimulating factor (M-CSF) expression through nuclear factor kappaB (NF-kappaB) activation, and treatment of human pancreatic MIA PaCa-2 cancer cells with forskolin or cAMP attenuated the NF-kappaB activation as well as M-CSF expression. In this study, we have further investigated the mechanism of c...

Forskolin is an extract of the Coleus forskholii plant that is widely used in cell physiology to raise intracellular cAMP levels. In the field of trophoblast biology, forskolin is one of the primary treatments used to induce trophoblastic cellular fusion. The syncytiotrophoblast (ST) is a continuous multinucleated cell in the human placenta that separates maternal from fetal circulations and ca...

Acquiredresistance to chemotherapeutic agents is an importantclinical problem. One preclinical model, termed multidrug resistance (MUR), is characterized by a complex phenotype of cross-resistance to biochemi cally unrelated antineoplastic agents, the presence of a high-molecularweight membrane glycoprotein, and impaired accumulation of drug. To determine whether MDR is mediated in part by alte...

Regulated urine concentration takes place in the renal collecting duct upon arginine vasopressin (AVP) stimulation, where subapical vesicles containing aquaporin-2 (AQP2) are inserted into the apical membrane instantly increasing water reabsorption and urine concentration. The reabsorped water exits via basolateral AQP3 and AQP4. Upon long-term stimulation with AVP or during thirst, expression ...

Cui, Xun, Jin Fu Wen, Jing Yu Jin, Wen Xie Xu, Sung Zoo Kim, Suhn Hee Kim, Ho Sub Lee, and Kyung Woo Cho. Protein kinase-dependent and Ca2 -independent cAMP inhibition of ANP release in beating rabbit atria. Am J Physiol Regulatory Integrative Comp Physiol 282: R1477–R1489, 2002; 10.1152/ajpregu.00316.2001.—Regulation of atrial release of atrial natriuretic peptide (ANP) is coupled to changes i...

Synaptic facilitation at sensory-to-motor neuron synapses in Aplysia is a mechanism contributing to a simple form of learning called behavioral sensitization. Previous work has shown that facilitation is mediated in part by the neurotransmitter 5-HT acting through cAMP to broaden presynaptic action potentials and thus increase transmitter release from the sensory neuron terminals. Other studies...