The fractionation of stable isotopes in aquatic environments has been used extensively to track the movement of elements through aquatic food webs [e.g. (Stapp et al., 1999; Zanden et al., 1999)] and to understand ecological processes (Lajtha and Michener, 1994). In particular, the changing rate of stable isotope fractionation of biological and physical processes due to changing environmental c...

A wide range of abstract characteristics of partitions have been proposed for cluster evaluation. We empirically evaluated the performance of these metrics for flow cytometry data and found that the set-matching metrics perform closest to human.

The correlation between stress-induced nucleolar disruption and abrogation of p53 degradation is evident after a wide variety of cellular stresses. This link may be caused by steps in p53 regulation occurring in nucleoli, as suggested by some biochemical evidence. Alternatively, nucleolar disruption also causes redistribution of nucleolar proteins, potentially altering their interactions with p...

An altered state of cell hydration is a hallmark of a number of RBC disorders. The two parameters most often used to characterize the state of cell hydration are (a) the complete cell density distribution profile, and (b) the fraction of cells with densities greater than a defined value. Buoyant density cell fractionation with a variety of polymers has long been the preferred method for obtaini...

In a single round: By combining the high-efficiency enrichment through the continuous-flow magnetic separation (CFMS) technique with the analytical power of next-generation sequencing, the generation of antibody mimetics with a single round of mRNA display is made possible. This approach eliminates iterative selection cycles and provides a path to fully automated ligand generation (see picture).

two experiments were conducted to evaluate the crude protein fractionation, in vitro gas production and the biological effects of tannins in alfalfa silage treated with different levels of pistachio by-products (pb) extract. pb was soaked in water with a ratio of 1:5 (w/v) for 12 h. after filtering and concentrating, the crude extract was added to fresh alfalfa to a final concentration of 1%, 2...

Salvia genus is one of the largest genera of the Lamiaceae family. Its species have been used for a wide variety of disorders in the local traditional medicine systems. Therefore, the genus has been the subject of several phytochemical and biological studies. The aim of the study was to identify the major antioxidant compound(s) from the methanol extract of Salvia verticillata using activity-gu...

CTFFIND is a widely-used program for the estimation of objective lens defocus parameters from transmission electron micrographs. Defocus parameters are estimated by fitting a model of the microscope's contrast transfer function (CTF) to an image's amplitude spectrum. Here we describe modifications to the algorithm which make it significantly faster and more suitable for use with images collecte...