FRAP has been used to quantify the mobility of GFP-tagged proteins. Using a strong excitation laser, the fluorescence of a GFP-tagged protein is bleached in the region of interest. The fluorescence of the region recovers when the unbleached GFP-tagged protein from outside of the region diffuses into the region of interest. The mobility of the protein is then analyzed by measuring the fluorescen...

Orphanides A., Goulas V., Gekas V. (2013): Effect of drying method on the phenolic content and antioxidant capacity of spearmint. Czech J. Food Sci., 31: 509–513. The changes in total phenolics, hydroxycinammic acid derivatives, and antioxidant properties of spearmint after five drying treatments (convection oven drying, freeze-drying, microwave drying, and air drying with the sun exposure and ...

BACKGROUND Retention of nutrients in vegetables during blanching/freezing, cooking and warm-holding is crucial in the preparation of both standard and therapeutic diets. In the present study, conventional cooking in water, and cooking by pouch technology (boil-in-bag, sous vide) were compared in their ability to retain vitamin C, total phenolics and antioxidative activity (DPPH and FRAP) in ind...

BACKGROUND Mad1 and Mad2 are constituents of the spindle-assembly checkpoint, a device coupling the loss of sister-chromatid cohesion at anaphase to the completion of microtubule attachment of the sister chromatids at metaphase. Fluorescence recovery after photobleaching (FRAP) revealed that the interaction of cytosolic Mad2 with kinetochores is highly dynamic, suggesting a mechanism of catalyt...

BACKGROUND Podophyllum hexandrum Royle (P. hexandrum) is a perennial herb and widely used in clinic. The present study was designed to separate and identify the biochemical composition and antioxidant capacity of extracts from P. hexandrum rhizome. METHODS The ethyl acetate and ethanol extracts from P. hexandrum rhizome were analyzed by GC-MS (gas chromatography-mass spectrometry), and the an...

objectives: the antioxidant capacity impairs in kidney and urinary bladder of animals with stone disease. herbal medicine can improve the antioxidant condition of renal tissue. cynodon dactylon (c. dactylon) is a medicinal plant with antioxidative and diuretic properties and different preparations of this plant have shown promising effects in stone disease. assessment of the whole plant decocti...

human milk contains all of the constituents that are required for the optimal growth and development of a neonate. it supports the development of brain, immune, and physiological systems. this study aimed to consider the significance of breast milk in preventing oxidative stress by comparing total antioxidant capacity (tac) in breast and formula milk for premature infants, demonstrating the rel...

herbal medicines can be used as the potential sources of anti-oxidative compounds to help the treatment of diseases associated to oxidative stress. in this paper, the ferric reducing antioxidant power (frap) activity of four lamiaceae herbal extracts, which traditionally applied in oxidative stress related diseases, has been evaluated and total phenolics contents of these extracts determined by...