AIMS To assess protective efficacy of genetically modified Escherichia coli Nissle 1917 (EcN) on metabolic effects induced by chronic consumption of dietary fructose. MATERIALS AND METHODS EcN was genetically modified with fructose dehydrogenase (fdh) gene for conversion of fructose to 5-keto-D-fructose and mannitol-2-dehydrogenase (mtlK) gene for conversion to mannitol, a prebiotic. Charles ...

One-compartment biofuel cells without separators have been constructed, in which d-fructose dehydrogenase (FDH) from Gluconobacter sp. and laccase from Trametes sp. (TsLAC) work as catalysts of direct electron transfer (DET)-type bioelectrocatalysis in the two-electron oxidation of d-fructose and four-electron reduction of dioxygen as fuels, respectively. FDH adsorbs strongly and stably on Ketj...

We present the two-loop QCD helicity amplitudes for quark-gluon scattering, and for quark-antiquark annihilation into two gluons. These amplitudes are relevant for next-to-next-to-leading order corrections to (polarized) jet production at hadron colliders. We give the results in the ‘t Hooft-Veltman and four-dimensional helicity (FDH) variants of dimensional regularization. The transition rules...

Aldehyde biosensors were constructed by cross-linking formaldehyde dehydrogenase (FDH) or aldehyde dehydrogenase (ADH) and bovine serum albumin on the surface of a redox Os-polymer-coated electrode. The prepared aldehyde biosensors responded rapidly (within 30 s) to aldehydes without the addition of a soluble mediator, because the inner redox Os-polymer film effectively mediated the electron tr...

The fructose/dioxygen biofuel cell, one of the direct electron transfer (DET)-type bioelectrochemical devices, utilizes fructose dehydrogenase (FDH) on the anode and multi-copper oxidase such as bilirubin oxidase (BOD) on the cathode as catalysts. The power density in the literature is limited by the biocathode performance. We show that the DET-type biocathode performance is greatly improved, w...

Cotyledons and hulls were prepared from twelve varieties of field beans (Vicia faba L.). Adult cockerels were tube-fed either beans, cotyledons or hull diets containing high or low levels of protein. Metabolizable energy coefficients and starch digestibility coefficients were determined for beans, cotyledons and hull diets. Lipid digestibility coefficients from hull diets were also determined. ...

Depth cameras, which provide color and depth information per pixel at video rates, offer exciting new opportunities in computer graphics. We address the challenge of supporting free-viewpoint video of dynamic 3D scenes using live data captured and streamed from widely-spaced viewpoints by a handful of synchronized depth cameras. We introduce the concept of the depth hull, which is a generalizat...

The C-terminal domain (C(t)-FDH) of 10-formyltetrahydrofolate dehydrogenase (FDH, ALDH1L1) is an NADP(+)-dependent oxidoreductase and a structural and functional homolog of aldehyde dehydrogenases. Here we report the crystal structures of several C(t)-FDH mutants in which two essential catalytic residues adjacent to the nicotinamide ring of bound NADP(+), Cys-707 and Glu-673, were replaced sepa...