Fatty acid synthase (FASN) is the metabolic enzyme responsible for the endogenous synthesis of the saturated long-chain fatty acid palmitate. In contrast to most normal cells, FASN is overexpressed in a variety of human cancers including cutaneous melanoma, in which its levels of expression are associated with a poor prognosis and depth of invasion. Recently, we have demonstrated the mitochondr...

The transfer across the swine uterus and placenta of [1-14C]octanoic acid, [9,10(n)-3H]palmitic acid and [1-14C]linoleic acid was studied in five gilts and their fetuses during late gestation following a single bolus injection. Only trace amounts of labeled fatty acids were found in fetal plasma lipid. The highest fetal plasma lipid values were less than 1.2% of the peak maternal values. The co...

A number of studies have reported that a high-fat diet induces increases in mitochondrial fatty acid oxidation enzymes in muscle. In contrast, in two recent studies raising plasma free fatty acids (FFA) resulted in a decrease in mitochondria. In this work, we reevaluated the effects of raising FFA on muscle mitochondrial biogenesis and capacity for fat oxidation. Rats were fed a high-fat diet a...

The main function of white adipose tissue is to store nutrient energy in the form of triglycerides. The mechanism by which free fatty acids (FFA) move into and out of the adipocyte has not been resolved. We show here that changes in intracellular pH (pH1) in adipocytes correlate with the movement of FFA across cellular membranes as predicted by the Kamp and Hamilton model of passive diffusion o...

Free fatty acids (FFA), the uranyl ion, and the basic dye Rhodamine B form colored complexes, which are extractable into toluene or benzene. Fatty acids of different chain lengths above C(10) and different degrees of unsaturation gave constant molar yield. Complexes in toluene alone are unstable, especially in the light, but a small amount of aqueous uranyl acetate stabilizes them sufficiently ...

Microalgae are photosynthetic microorganisms with high lipid content, capable of degrading nutrients from wastewater. In this research, two strains microalgae, Scenedesmus sp. and Chlorella vulgaris were cultivated in sterilized pig slaughterhouse wastewater using outdoor flat photobioreactors. Cell growth, total lipids, free fatty acids (FFA), acid methyl esters (FAME) physicochemical paramete...

BACKGROUND In vitro exposure of liver cells to high concentrations of free fatty acids (FFA) results in fat overload which promotes inflammatory and fibrogenic response similar to those observed in patients with Non-Alcoholic Fatty Liver Disease (NAFLD) and Non-Alcoholic Steatohepatitis (NASH). Since the mechanisms of this event have not been fully characterized, we aimed to analyze the fibroge...

This study aimed to investigate the oxidation profile of wheat germ oil extracted from raw during stabilization with microwave (MW) treatment, and kinetics parameters (free fatty acids (FFA), peroxide value (PV), thiobarbituric acid (TBA), α-tocopherol, lipase (LA) lipoxygenase (LOX) enzymes activities) under different storage conditions. For stabilizing germ, MW was treated at 700 W for three ...

fatty acids play important roles in biological systems and the newborns fatty acids requirements are covered only by the milk. it is of particular interest to qualify the content of the fatty acids in the milk. this study was performed to determine the levels of some fatty acids in the infant formulas and also to describe a method without derivatization for the fatty acids analysis and applying...

Familial Mediterranean fever (FMF) is an autosomal recessive disease of unknown etiology and has no known diagnostic markers. Periodic attacks of pain and fever can be precipitated by dietary fat or dairy products and by the same factors that are known to elevate serum free fatty acids (FFA). Several tests related to lipid metabolism were made on the serum and urine of a fraternal twin with FMF...