This study was prompted by the observation that fresh platelet suspensions--prepared by gel filtration or albumin density gradient centrifugation--possessed only minimal factor V activity, whereas frozen-and-thawed platelet suspensions possessed striking factor V activity. Results of experiments with fresh suspensions suggested that unaltered platelets did not bind plasma factor V. The factor V...

The value of the ability to cryopreserve and store germplasm has long been recognized for indefinite preservation of genetic material, especially for at-risk populations. In contrast to domestic livestock species, cryogenic storage of poultry semen is not reliable enough for germplasm preservation. The relatively low fertilizing ability of frozen/thawed poultry sperm most likely results from ph...

OBJECTIVE To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. DESIGN Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. SETTING University-affiliated laboratory. ANIMALS Rhesus monkey ovarian tissue. INTERVENTION(S) No...

OBJECTIVE This study aims to evaluate the effectiveness of GnRH agonist in comparison with hCG for triggering final oocyte maturation in endometrial preparation of letrozole stimulation protocols for frozen-thawed embryo transfer. METHODS The frozen-thawed embryo transfer cycles (FET) that use the letrozole stimulation protocols for endometrial preparation were divided into two groups accordi...

The present study was conducted to evaluate the in vivo fertility of frozen thawed boar semen. Twenty ejaculates collected from six mature boars were frozen in a programmable freezer. After freezing the semen was evaluated for different sperm quality parameters. Twenty five sows were inseminated artificially utilizing frozen thawed semen. The percentage of sperm motility, live sperm, live intac...

BACKGROUND Ectopic pregnancy (EP) occurs in 1% of pregnancies and is reported to be more common in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) pregnancies. An abdominal ectopic pregnancy (AEP) is a rare form of EP, and there are few reports of an AEP after IVF/ICSI. In this case report, a rare case of AEP after frozen-thawed cycle of ICSI is presented. CASE PRESENTATION...

Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructoseglycerol extender 1:4) and thawed (40 0 C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 25 0 C up to 5 0 C) and equilibrated (at 5 0 C for 2 hours) semen straws were frozen @ 15, 20, 25 and 30 0 C/minute for M1, M2, M3 and M4 groups respectively. These frozen str...

The objective of this study was to assess whether the exposure of zona pellucida-intact bovine embryos to the proteolytic enzyme, trypsin, during embryo washing has a detrimental effect on their subsequent survival and development. Embryos were collected nonsurgically from superovulated cows (n = 19) 7.5 d after insemination. Grade 1 and Grade 2 embryos were washed 12 times in modified Dulbecco...