Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy using a special waveguide based on a silver halide fiber was used for probing the heat-induced secondary structure and conformation changes of bovine serum albumin (BSA). From the secondary derivative and the curve fitting of the obtained ATR-FTIR spectra, the changes of the BSA secondary structure with temperature w...

background & aim: allium sativum commonly known as garlic is an herb used over years to prevent from various ailments such as cardiovascular diseases, cognitive disorders and also enhances the immune system. the aim of the present study is to study the in vitro antiarthritic and antihemolytic potential of garlic in aqueous and methanolic extracts. fourier transform infrared spectroscopy was per...

metronidazole is used to treat trichomoniasis, bacterial vaginosis, and other diseases. there are controversy aspects about its teratogenicity. a teratogenic agent can alter morphology or subsequent function of the fetus. the aim of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by metronidazole usin...

evaluation of pre-fixed biological tissues preparation methods for atr-ftir biospectroscopy fourier transform infrared (ftir) spectroscopy in atr (attenuated total reflection) mode is a powerful tool for studying biomedical samples, which can provide important structural information on the molecular composition. currently, formalin fixation and paraffin preservation (ffpp) is the preferred source ...

A spectrophotometer based on an integrating sphere coupled to a commercial Fourier Transform Infrared (FTIR) instrument has been constructed for regular spectral transmittance and re ectance measurements over the 1 m m to 18 m m wavelength region. Despite the large number of sources of error that often limit the radiometric accuracy of FTIR measurements, we demonstrate uncertainties similar to...

Phototropin (Phot), a blue-light photoreceptor in plants, consists of two FMN-binding domains (named LOV1 and LOV2) and a serine/threonine (Ser/Thr) kinase domain. We have investigated light-induced structural changes of LOV domains, which lead to the activation of the kinase domain, by means of light-induced difference FTIR spectroscopy. FTIR spectroscopy revealed that the reactive cysteine is...