نتایج جستجو برای: functionalized single

تعداد نتایج: 890866  

Journal: :Lab on a chip 2013
Venkatachalam Chokkalingam Jurjen Tel Florian Wimmers Xin Liu Sergey Semenov Julian Thiele Carl G Figdor Wilhelm T S Huck

Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughp...

Journal: :BMC Biotechnology 2009
Sanne WA Reulen Ingrid van Baal Jos MH Raats Maarten Merkx

BACKGROUND Classical bioconjugation strategies for generating antibody-functionalized nanoparticles are non-specific and typically result in heterogeneous compounds that can be compromised in activity. Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester, providing a unique handle for site-specific conjugation using n...

Journal: :Lab on a chip 2011
Peyman Honarmandi Hyungsuk Lee Matthew J Lang Roger D Kamm

We present a new method to locally apply mechanical tensile and compressive force on single cells based on integration of a microfluidic device with an optical laser tweezers. This system can locate a single cell within customized wells exposing a square-like membrane segment to a functionalized bead. Beads are coated with extracellular matrix (ECM) proteins of interest (e.g. fibronectin) to ac...

Journal: :Advanced Healthcare Materials 2023

Sonothermal Therapy In article 2300291 by Junwei Ye, Ying Che, Guiling Ning, and co-workers, a novel “B–H” interfacialconfined coordination strategy is reported to synthesize hyaluronic acid-functionalized single copper atoms dispersed over boron imidazolate framework-derived nanocubes achieve sonothermal-catalytic synergistic therapy. This study highlights the applications of single-atomcoordi...

Journal: :Soft matter 2015
Samala Murali Mohan Reddy Ganesh Shanmugam Natarajan Duraipandy Manikantan Syamala Kiran Asit Baran Mandal

In recent years, several fluorenylmethoxycarbonyl (Fmoc)-functionalized amino acids and peptides have been used to construct hydrogels, which find a wide range of applications. Although several hydrogels have been prepared from mono Fmoc-functionalized amino acids, herein, we demonstrate the importance of an additional Fmoc-moiety in the hydrogelation of double Fmoc-functionalized L-lysine [Fmo...

Journal: :iranian chemical communication 2014
mohammad javad taghizadeh khosrow jadidi

an efficient one-pot three-component procedure for the synthesis of new chiral spiro oxindolopyrrolidines/pyrrolizidines with highly regio- and diastereo-enantio, selective from 1,3-dipolar cycloaddition of azomethine ylides and chiral menthol-drived trans-cinnamic are described. the mechanism of the reaction is discussed on basis of the assignment of the absolute configuration of one of the cy...

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