Different compositions of liquid media were used for their efficacy in the production of beta-galactosidase by Aspergillus oryzae in submerged fermentation. Enzyme production was improved by switching from lactose-based media to soybean meal- and wheat bran-based media. Further improvement was made when the soybean meal medium was supplemented with sodium nitrate and magnesium sulfate. Res...

The biotechnological potential of pectinolytic fungi isolated from citrus fruits was studied. Seventy five fungal isolates were isolated from decayed citrus fruits. All of these isolates were screened for their pectinolytic activity. Thirty six out of seventy five isolates proved to have pectinolytic activity. These isolates were identified and classified into twenty strains belonging to eleven...

Class I alpha1,2-mannosidases (glycosylhydrolase family 47) are key enzymes in the maturation of N-glycans. This protein family includes two distinct enzymatically active subgroups. Subgroup 1 includes the yeast and human endoplasmic reticulum (ER) alpha1,2-mannosidases that primarily trim Man(9)GlcNAc(2) to Man(8)GlcNAc(2) isomer B whereas subgroup 2 includes mammalian Golgi alpha1,2-mannosida...

Wood-decaying fungi in the phylum Basidiomycota play a significant role global carbon cycle, as they decompose deadwood effectively. Fungi may compete for utilizable substrate and growth space by producing soluble metabolites releasing volatile organic compounds (VOCs). We determined of wood (Scots pine or Norway spruce) on generation hyphal biomass, secreted enzyme activities, decomposition ra...

Twenty-one fungal species were screened for capability of removing pyrene and benzo(a)pyrene in culture. Xylaria regalis was found to show the highest activity. An PAHs-degrading enzyme was purified to homogeneity from the culture mycelium of X. regalis. The crude enzyme was subjected to a series of purification procedures, including QAE Sephadex A-25 anion exchange column, hydrophobic interact...

The fungal strain Humicola lutea 103 produces a naturally glycosylated Cu/Zn SOD. To improve its yield, the effect of an increased concentration of dissolved oxygen (DO) on growth and enzyme biosynthesis by the producer, cultivated in a 3 l bioreactor, was examined. Exposure to a 20% DO level caused a 1.7-fold increase of SOD activity compared to the DO-uncontrolled culture. Maximum enzyme prod...

Purpose Developing immunotherapies for fungal eye infections is a high priority. We analyzed fungal pathogens for expression of the surface polysaccharide, poly-N-acetyl glucosamine (PNAG), and used a mouse model of ocular keratitis caused by Aspergillus flavus, A. fumigatus, or Fusarium solani to determine if PNAG was an immunotherapy target and requirements for ancillary cellular and molecula...

BACKGROUND Chemical methods of producing dyes involve extreme temperatures and unsafe toxic compounds. Application of oxidizing enzymes obtained from fungal species, for example laccase, is an alternative to chemical synthesis of dyes. Laccase can be replaced by fungal biomass acting as a whole-cell biocatalyst with properties comparable to the isolated form of the enzyme. The application of th...

Innovative green technologies are of importance for converting plant wastes into renewable sources for materials, chemicals and energy. However, recycling agricultural and forestry wastes is a challenge. A solution may be found in the forest. Saprotrophic white-rot fungi are able to convert dead plants into consumable carbon sources. Specialized fungal enzymes can be utilized for breaking down ...

BACKGROUND The glucuronoyl esterase enzymes of wood-degrading fungi (Carbohydrate Esterase family 15; CE15) form part of the hemicellulolytic and cellulolytic enzyme systems that break down plant biomass, and have possible applications in biotechnology. Homologous enzymes are predicted in the genomes of several bacteria, however these have been much less studied than their fungal counterparts. ...