Although current interest in basidiomycete fungi is focused mainly on taxonomy and exploitation of selected species in environmental biotechnology, isolation and characterization of their nucleic acids is becoming still more and more important for many purposes. Differences in cell wall composition and the mode of growth of some basidiomycetes are the major limitations for utilization of common...

the eukaryotic complexity involves the expression and regulation of genes via rna-dna, rna-rna, dna-protein and rna-protein interactions. recently, the role of rna molecules in the regulation of genes in higher organisms has become more evident, especially with the discovery that about 97% of the transcriptional output in higher organisms are represented as noncoding rnas: rrna, snornas, trna, ...

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amplification and sequencing of relatively short DNA regions. For fungi, the internal transcribed spacer (ITS) region in the ribosomal RNA (rRNA) operon has been accepted as the formal fungal barcode. Despite an increasing number of fungal metabarcoding studies, the amplification efficiency of prime...

for study the genetic diversity and phylogenetic analysis of polymyxa betae keskin , isolated from sugar beet fields in razavi and northern khorasan provinces ,soil of 49 fields were sampled and sugar beet seeds (variety ic) were planted that .after dna extraction, a genomic region comprises of 463 bp of p.betae isolation including 18s r rna، its1 ,5.8srrna ،its2 and 28s rrna was amplified usin...

background and purpose: airborne fungi are one of the most important agents responsible for triggering allergic reactions such as rhinitis and severe asthma. this study was conducted to analyze and monitor the prevalence and distribution patterns of atmospheric fungal aerosols in the air of qazvin during winter of 2012. materials and methods: in the current descriptive study, the incidence and ...