1320 blood samples were collected from herds showing clinical signs of pestiviral infections. 39 samples were positive in ag-elisa assay. all these 39 samples in addition to 5 cytopathic strains were cultured in md-bk cell line and presence of pestiviral antigens was confirmed by direct-immunofluorescent test. 27 out of 44 bvdv suspected isolates were detected by rt-pcr using a primer set. diff...

background and aims: hepatitis b virus (hbv) infects the liver and causes acute and chronic hepatitis, hepatocellular carcinoma and cirrhosis. hbv has been divided to eight genotypes (a–h) and subgenotypes of a, b, c and f. for the first time, we determined hbv genotypes in infected samples by inno-lipa method in yazd, central province of iran. materials and methods: this study was performed on...

background hepatitis c virus (hcv) is the major cause of chronic liver disease. hcv is a single stranded positive sense rna of approximately 9.6 kb. because of high conservativeness of 5΄untranslated region of hcv genome, it is widely used for virus genotyping. different methods are used for the virus genotyping, but all involve some difficulties. objectives the aim of the present study was to ...

ObjectivesTo investigate the correlation between epidermal growth factor (EGF) polymorphism and risk of hepatocellular carcinoma (HCC) in hepatitis C viral (HCV)-related cirrhotic patients as well its relation to EGF protein expression HCC tissue.BackgroundIn Egypt, incidence is ∼4.7% chronic liver disease because HCV infection. plays an important role hepatocyte regeneration also a malignant t...

Background: The purpose of this study was to determine the profiles patients with type 2 diabetes (T2DM) and an SLC22A1 gene mutation in order evaluate effect metformin pharmacogenetics.
 Methods: To assess pharmacogenetics, a T2DM receiving investigated. Blood samples were taken from 50 diabetics Minangkabau ethnicity who met inclusion criteria, SNP genotyping blood glucose levels determi...

The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...

The purpose of this study was to examine the effects PPAR-a (rs4253778) on serum lipids in elite cross-country skiers. This included 34 skiers (23 males and 11 females who participated Turkish skiing national team camp). Genotyping for gene rs4253778 G/C polymorphism performed by PCR Tercyk multicanal amplificator restriction enzyme digestion. Statistical analysis done using SPSS 22.0 package p...

OBJECTIVE Genotyping for CYP2D6 has the potential to predict differences in metabolism of nortriptyline. This information could optimize pharmacotherapy. We determined the costs and effects of routine genotyping for old aged Dutch depressed inpatients. METHODS With a decision-tree, we modelled the first 12 weeks of nortriptyline therapy. Direct costs of genotyping, hospitalization, therapeuti...