The effects of iron deficiency and iron resupply on the metabolism of leaf organic acids have been investigated in hydroponically grown sugar beet. Organic acid concentrations and activities in leaf extracts of several enzymes related to organic acid metabolism were measured. Enzymes assayed included phosphoenol pyruvate carboxylase (PEPC; EC 4.1.1.31), different Krebs cycle enzymes: malate deh...

A leishmania parasite was isolated from Didelphis marsupialis aurita trapped in the municipality of Conchas in the state of São Paulo (YOSHIDA et al. 1979, Rev. Inst. Med. trop. São Paulo 21 (2 ) : 110-113). This isolate has now been analysed at the genotypic and phenotypic levels. Zymodeme analysis using agarose gel electrophoresis and 6 enzymes (ALAT, ASAT, MDH, G6PDH, GPI & PGM) showed that ...

The maturation and developmental competence of the oocyte is acquired during folliculogenesis. It is still unclear whether follicle size is associated with the levels of transcript and protein encoding molecules contributing to the fertilization ability of the porcine oocyte. Follicles were dissected from porcine ovaries after slaughter and classified as small (< 3 mm), medium (3-5 mm) or large...

Buprenorphine is now increasingly prescribed as an alternative to methadone for the treatment of heroin addiction. Because of its potency (dosage usages from 0.2 mg to 8 mg), the drug concentrations in body fluids are normally very low. Here, we report the first recombinant glucose-6-phosphate dehydrogenase (G6PDH)-based homogeneous immunoassay (EMIT-type assay) for free buprenorphine and free ...

The effects of heavy metals on growth, intermediary metabolism and enzyme activities were investigated in yellow perch (Perca fla escens), sampled in summer and fall from lakes situated along a contamination gradient of Cd, Zn and Cu in the mining region of Rouyn–Noranda, Québec. An exposure-dependent decrease in condition factor was observed in both seasons. Liver glycogen and triglyceride res...

" The reasonable man adapts himself to the world; the unreasonable one persists in trying to adapt the world to himself. Therefore, all progress depends on the unreasonable man. " III Börjesson A and Carlsson C. Altered proinsulin conversion in rat pancreatic islets exposed long-term to various glucose concentrations or interleukin-1ȕ. regulation of the expression of TNF-Į, IFN-Ȗ and IL-10 by s...

The use of biomarkers has become an important tool for modern environmental assessment as they can help to predict pollutants involved in the monitoring program. Here I present data on bioaccumulation, biochemical and tissues biomarkers in Nile tilapia as early warning indicators of river Nile pollution. Nile tilapia sampled from downstream sites accumulated higher levels of all the detected he...

This work presents the first known use of response surface methodology (RSM) in electrophoretically mediated microanalysis. This concept is demonstrated by examining the optimization of reaction conditions for the conversion of nicotinamide adenine dinucleotide to nicotinamide adenine dinucleotide, reduced form by glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) in the conversion of gluco...

The energy metabolism of cardiac hypertrophy in spontaneously hypertensive rats (SHR) was studied chronologically by histochemical and in part chemical methods. The activities of various enzymes, such as glucose-6-phosphate dehydrogenase (G6PDH), lactate dehydrogenase (LDH), isocitrate dehydrogenase, succinate dehydrogenase, beta-hydroxybutylate dehydrogenase (beta-HBDH) and monoamine oxidase (...

l h e onset of photosynthetic NO3assimilation in N-limited Chlamydomonas reinhardtii increased the initial extractable activity of the glucose-6-phosphate dehydrogenase (C6PDH), the key regulatory step of the oxidative pentose phosphate pathway. l h e total activated enzyme activity did not change upon NO3resupply. l h e higher activity, therefore, represents activation of existing enzyme. No a...