Two classes of 7S gamma-globulins are identified in normal mouse serum and are designated 7S gamma(2)-globulins and 7S gamma(1)-globulins. These two immunoglobulin classes are similar in size. They differ in range of electrophoretic mobility, in specific antigenic determinants, and in genetically determined isoantigens. Four classes of immunoglobulins in mouse serum, i.e. 7S gamma(2)-, 7S gamma...

Serum electrophoresis was done in 31 patients with pulmonary tuberculosis and 62 age and sex matched controls. Protein values amongst controls were higher than those described by others. In tuberculosis there was significant decrease in total proteins, and albumin with corresponding increase in globulin, mainly due to an increase in gamma globulin fraction. There was no significant increase in ...

Biologically active preparations of 125I-thyrotropin, [3H]thyrotropin, and the [3H]exophthalmogenic factor derived from thyrotropin by partial pepsin digestion have been used to study the binding properties of the thyrotropin receptor on guinea pig retro-orbital tissue plasma membranes. In regard to the optimal conditions of binding, pH, buffer, salt concentrations, and temperature, these prope...

The nature of the antibodies produced by the rabbit during the primary and secondary responses to T(2) phage, proteins, and the O and H antigens of Salmonella typhosa has been determined. Immune sera have been fractionated by zone electrophoresis, sucrose density ultracentrifugation, and anion exchange chromatography. The resulting fractions have been assayed by phage neutralization or hemagglu...

Diffuse glomerulonephritis was induced in unilaterally nephrectomized albino rabbits by giving them two massive intravenous injections of purified bovine serum gamma globulin. Morphologically, these renal lesions were similar to those of human acute and subacute glomenilonephritis. Globulin nephritis in rabbits also closely resembles experimental nephritis induced by injection of other foreign ...

An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acet...

The plasma protein pattern of patients with uncomplicated essential hypertension showed only slight variations from the normal while that of patients with severely malignant hypertension showed marked shifts. The fibrinogen and beta-globulins were usually elevated beyond the normal range and the albumin decreased. In less severely malignant hypertension, the changes were less marked. In dogs wi...

The globulin content of the standard solution in the vials is standardized either by a Kjeldahl or a biuret determination. When available Lederle (gamma) globulin diluted 1:200 can be used for this purpose as well. The diluted Lederle standard globulin coagulates on heating and so cannot be used as a sterilized standard. An interesting phenomenon was observed while preparing the sterilized wate...

BACKGROUND Accelerated matrix breakdown caused by the increased activity of matrix metalloproteinases (MMPs) and/or the quantitative imbalance between MMP and tissue inhibitor of MMP (TIMP) have been implicated in several pathological conditions. MMP and TIMP may also be involved in the destruction of the coronary arterial wall and the resultant coronary arterial lesions in Kawasaki disease. ...

HE PROTEINS OF NORMAL HUMAN SERUM have been resolved both by moving-boundary and filter-paper electrophoresis chiefly into five components: albumin, , , , and’ globulins. In various disease states, by both technics, other protein components have been described. A component migrating between 22 globulin and beta globulin has been observed by Block et al. (1) in primary systemic amyloidosis and w...