The defense system of the skin has three layers: barrier, innate immunity, and acquired immunity. Epidermal keratinocytes participate in all these processes play central roles inflammatory responses. However, a shared molecule that responds to diverse dangers governs protective machinery remains obscure. Here we report orphan gene C10orf99 plays primary role response barrier formation skin. pep...

The activity of ribosomal gene clusters has been studied by cytological methods in human cultured cells grown in different amounts of serum under controlled experimental conditions. It has been shown that increasing amounts of serum induce an increase in ribosomal RNA synthesis at the single cell level. Furthermore, the concomitant identification of individual rRNA gene clusters by fluorescent ...

Lactose synthesis is believed to be rate limiting for milk production. However, understanding the molecular events controlling lactose synthesis in humans is still rudimentary. We have utilized our established model of the RNA isolated from breast milk fat globule from seven healthy, exclusively breastfeeding women from 6 h to 42 days following delivery to determine the temporal coordination of...

Abstract Background Acinetobacter baumannii is a common nosocomial pathogen that poses huge threat to global health. Owing the severity of A. infections, it became necessary investigate epidemiological characteristics in Chinese hospitals and find reasons for high antibiotic resistance rate mortality. This study aimed epidemiologic genetic isolated from patients with hospital acquired pneumonia...

Etioplasts of 4.5-day-old dark-grown barley synthesize and accumulate most of the membrane and nearly all the soluble polypeptides of mature chloroplasts of light-grown seedlings. Etioplasts do not synthesize a limited set of chloroplast-encoded polypeptides which are major constituents of chloroplast thylakoid membranes: two chlorophyll apoproteins of photosystem I (68 and 65 kDa), two chlorop...

Gene Fabrication technology involves the development and optimization of methods relevant to the in vitro synthesis of any given target gene sequence(s) in the absence of template. The driving purpose of this field of research is to bring about the capability for on-demand fabrication of a DNA construct of arbitrary length and sequence quickly, efficiently, and cost-effectively. The first part ...

Interleukin-13 (IL-13) belongs to the IL-4 gene family. Like IL-4, IL-13 induces IL-1 receptor antagonist (IL-1Ra) synthesis with no effect on IL-1beta synthesis. We investigated whether IL-13 induces IL-1Ra synthesis via a pathway similar to IL-4. In human peripheral blood mononuclear cells, IL-13 (1 to 100 ng/mL alone induced IL-1Ra synthesis in a dose-dependent manner. A single amino acid mu...

Abstract The Modern Synthesis (MS) gene-centered population model of evolution is currently being challenged by the Extended Evolutionary (ESS) organism-centered developmental evolution. predictions EES are here examined with respect to arguments Edward Drinker Cope (1840–1897) for an evolutionary process in which organisms both shape and shaped their environments such that activities themselve...

Interference RNAs are non-coding produced naturally o synthetically that temporarily inhibit the gene expression of specific genes. With this technology, it is possible to introduce a siRNA molecule against mRNA into cells and reduce synthesis protein encoded on mRNA. This novel strategy has been used by several researchers worldwide in medical area as treatment many hereditary acquired diseases.

An expression vector containing the gene encoding most common isoform Bet v 1.0101 of 1 protein, major birch pollen allergen, was created for a subsequent in prokaryotic system Escherichia coli. Total RNA from collected Belarus used as matrix. The pJC40-Веt obtained using molecular-genetic methods: cloning, ligation, transformation. specificity cloned fragment confirmed by sequencing. During st...