This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that...

Background; Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes Immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve ...

Experimental results show that specifi c binding between a ligand and surface immobilized receptor such as hybridization of single-stranded DNA (ssDNA) immobilized on a microcantilever surface leads to cantilever defl ection. The binding induced defl ection may be used as a method for detection of biomolecules, such as pathogens and biohazards. Mechanical deformation induced because of hybridiz...

AIM To explore the influence of Escherichia coli on the motility of human spermatozoa and its possible mechanism. METHODS Highly motile preparations of spermatozoa from normozoospermic patients were coincubated with Escherichia coli for 4 hours. At 1, 2 and 4 hours of incubation, sperm motility was determined. The factor responsible for sperm immobilization without agglutination was isolated ...

Prolonged immobilization depresses insulin-induced glucose transport in skeletal muscle and leads to a catabolic state in the affected areas, with resultant muscle wasting. To elucidate the altered intracellular mechanisms involved in the insulin resistance, we examined insulin-stimulated tyrosine phosphorylation of the insulin receptor beta-subunit (IR-beta) and insulin receptor substrate (IRS...

An ideal head-immobilization method provides a high level of accuracy and reproducibility in the immobilization. Various head-immobilization methods for radiotherapy have been published and are excellent in terms of accuracy; however, these methods are complicated to use and labor intensive. This study describes 2 new bite-block-type head-immobilization devices designed for greater stability an...

Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), anima...

Several cultures of gram-negative and gram-positive bacteria were successfully immobilized with titanous hydroxide. The immobilization efficiency for the microorganisms investigated in saline and broth media ranged from 80.2 to 99.9%. The immobilization of salmonellae was effective over a wide pH range. The presence of buffers, particularly phosphate buffer, drastically reduced the immobilizati...