Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transf...

Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature e...

and the bar gene was transmitted through both male and female gametes and expressed in T1 progeny. Although Agrobacterium tumefaciens has been successfully used Microprojectile bombardment and gene transfer by to transfer genes to a wide range of plant species, it has received litA. tumefaciens are currently the two most common tle attention for transformation of forage grasses. Therefore, the ...

Studies show that gene acquisition through natural transformation has contributed significantly to the adaptation and ecological diversification of several bacterial species. Relatively little is still known, however, about the prevalence and phylogenetic distribution of organisms possessing this property. Thus, whether natural transformation only benefits a limited number of species or has a l...

Agrobacterium tumefaciens is a Gram-negative soil phytopathogenic bacterium that causes the crown gall disease of dicotyledonous plants, which is characterized by a tumorous phenotype. It induces the tumor by transferring a segment of its Ti plasmid DNA (transferred DNA, or T-DNA) into the host genome and genetically transforming the host. One century has past after A. tumefaciens was firstly i...

This review focus on Arachis functional and structural genomic studies, and their application in peanut improvement via plant transformation, that constitutes one of the most promising strategies to advance the introgression and modulation of important traits which can be translated into crop gain.

Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells bound equivalent amounts of [32P]DNA in a DNase-resistant state, no donor DNA...

HE science of genetics has undergone a tremendous development as a result T of the discovery of novel systems of exchange of genetic material. The first new system to be discovered was that of DNA-induced bacterial transformation (AVERY, MACLEOD and MCCARTY 1944) , yet as a purely genetic tool this system has been little used, in spite of possessing unique experimental possibilities. The presen...