Abstract With modern genetic engineering tools, microorganisms can become resilient green cell factories to produce sustainable biofuels directly. Compared non-engineered algae and cyanobacteria, the photon conversion efficiency be significantly increased. Furthermore, simplified harvesting processes are feasible since novel excreting or their precursors continuously directly into cultivation m...

Immunotherapy with genetically engineered T-cells that express the chimeric antigen receptor (CAR) has raised hopes for the treatment of pediatric malignancies. Although CAR T-cell development is on a fast-moving pace and evolution, the context of exploring novel targetable antigens has been neglected. In this review study, we analyze the prominent hematologic antigens targeted by engineered T-...

The use of intravascular stents may be limited by both local thrombosis and restenosis due to intimal proliferation. In an effort to provide solutions to these problems, we seeded stents with genetically engineered endothelial cells in vitro. Using retroviral-mediated gene transfer, we inserted the gene for either bacterial beta-galactosidase or human tissue-type plasminogen activator (t-PA) in...

Modularity the notion that individual components can be described as self-contained units and composed in different combinations is a concept integral to synthetic biology. In vitro, it is embodied by the notion of BioBrickparts: standardised DNA sequences of defined structure, function, and common interfaces that can be composed and integrated into living cells. A similar modularity in the mod...

In this report, we have investigated the relationship between androgen levels and prostate tumorigenesis in Nkx3.1; Pten mutant mice, a genetically engineered mouse model of human prostate cancer. By experimentally manipulating serum levels of testosterone in these mice for an extended period (i.e., 7 months), we have found that prolonged exposure of Nkx3.1; Pten mutant mice to androgen levels ...

Early detection represents one of the most promising approaches to improving lung cancer survival. To date, no screening strategies have been shown to decrease mortality from the disease. Furthermore, no reliable circulating biomarkers of lung cancer have been identified that allow early diagnosis. With the advent of gene targeting technology, new genetically engineered mouse models of lung can...

Terraforming of a planetary body (planet or moon) or planetary ecosynthesis is the hypothetical process of deliberately modifying its atmosphere composition, temperature, topography, or ecology to be similar to those of Earth to make it habitable for Terran organism, including humans. Terraforming is a common concept in science fiction. In fact, Jack Williamson, a science fiction writer, coined...

A considerable effort is currently underway to develop a malaria vaccine based on live Plasmodium falciparum sporozoites. The first requisite of a sporozoite vaccine is the guarantee of parasite arrest prior to the onset of the pathogenic blood stage. Immunisation with genetically attenuated parasites (GAP) that arrest in the liver forms a promising approach. Work in this thesis describes the d...

On May 8, 2014, Vermont passed the Vermont Genetically Engineered Food Labeling Act (Act) requiring labels on certain genetically engineered foods. Once the bill takes effect July 1, 2016, all Vermont-retailed foods with more than 0.9% of their total weight in genetically modified ingredients must be labeled with language stating, "may be partially produced with genetic engineering." As genetic...

Genetic modification of microorganisms for enhanced metabolic activity shows excellent promise biotechnological exploitation. The production solar fuels can be facilitated by novel advances in the engineering microalgae and cyanobacteria. Contaminant-free biocatalytic with high cell densities at a large scale low costs need to achieved commercial application this innovation from technical persp...