We explore the roles of gibberellin (GA) signaling genes SLEEPY1 (SLY1) and RGA-LIKE2 (RGL2) in regulation of seed germination in Arabidopsis thaliana, a plant in which the hormone GA is required for seed germination. Seed germination failure in the GA biosynthesis mutant ga1-3 is rescued by GA and by mutations in the DELLA gene RGL2, suggesting that RGL2 represses seed germination. RGL2 protei...

Seed germination is the most important transitional event between early stages in the life cycle of spermatophytes and understanding it is crucial to understand plant adaptation and evolution. However, so far seed germination of phylogenetically closely related species has been poorly investigated. To test the hypothises that phylogenetically related plant species have similar seed ecophysiolog...

This study was carried out in the biotechnology laboratory of Agricultural Faculty of Ondokuz May s University. The objective of this study was to establish the effects of chilling period and chilling temperature on germination in linseed (Linum usitatissimum L.). Seeds were subjected to six different heat temperatures (0, 2, 4, 6, 8 and 10 C) for 24, 48, 72, 96, and 120 hours. Germination test...

Using high-resolution 13C nuclear magnetic resonance, we examined the mobilization of endogenous trehalose in suspensions of yeast asci. Sporulation of yeast cells in [1-13C]acetate resulted in incorporation of label into the C-3 and C-4 positions of trehalose within the asci. During germination of these asci with [1-13C]glucose, the consumption of both endogenous trehalose and exogenous glucos...

The impact of natamycin on Aspergillus niger was analysed during the first 8 h of germination of conidia. Polarisation, germ tube formation, and mitosis were inhibited in the presence of 3 and 10 μM of the anti-fungal compound, while at 10 μM also isotropic growth was affected. Natamycin did not have an effect on the decrease of microviscosity during germination and the concomitant reduction in...

Uromyces appendiculatus is a rust fungus that causes disease on beans. To understand more about the biology of U. appendiculatus, we have used multidimensional protein identification technology to survey proteins in germinating asexual uredospores and have compared this data with proteins discovered in an inactive spore. The relative concentrations of proteins were estimated by counting the num...

Timely harvest is critical to achieve maximum seed viability and vigour in agricultural production. However, little information exists concerning how to reap the best quality seeds of Leymus chinensis, which is the dominant and most promising grass species in the Songnen Grassland of Northern China. The objective of this study was to investigate and evaluate possible quality indices of the seed...

Phelipanche aegyptiaca is one of the most destructive root parasitic plants of Orobanchaceae. This plant has significant impacts on crop yields worldwide. Conditioned and host root stimulants, in particular, strigolactones, are needed for unique seed germination. However, no extensive study on this phenomenon has been conducted because of insufficient genomic information. Deep RNA sequencing, i...

The changes in crude protein, free amino acids, amino acid composition, protein solubility, protein fractionation and protein digestibility after germination of sorghum were investigated. Sorghum varieties (Dorado, Shandaweel-6, Giza-15) were soaked for 20 h followed by germination for 72 h; the results revealed that crude protein and free amino acids in raw sorghum varieties ranged from 10.62 ...

The objective of the this study was to compare concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L(-1) with bulk TiO2 for phytotoxic and stimulatory effects on fennel seed germination and early growth stage. After 14 d of seed incubation, germination percentage highly improved following exposure to 60 ppm nanosized TiO2. Similar positive effects occurred in terms of shoot dry weigh...