The efficiency of 2 enzyme complexes on male broiler nutrient digestibility and performance were evaluated. A total of 120 and 840 day-old male broilers (Cobb 500 strain) were used in the metabolic and performance tests, respectively. The birds were distributed into 4 treatments [positive control (PC), negative control (NC), NC + enzyme complex A (xylanase, glucanase, amylase, protease and cell...

Biological control plays a crucial role in grapevine pathogens disease management. The cell-wall degrading enzymes chitinase, cellulase and β-glucanase have been suggested to be essential for the mycoparasitism activity of Trichoderma species against grapevine fungal pathogens. In order to develop a useful strain as a single source of these vital enzymes, it was intended to incorporate the char...

The autolytic release of fl-fructofuranosidase (EC 3.2.1.26) and acid phosphatase (EC 3.1.3.2) from the cell wall of yeast was studied under different organic solvents and with a variety of pII and temperature conditions. /3Fructofuranoeidase (but not acid phosphatase) was found to be a convenient marker for studying changes in the wall structure. The ethyl acetate-treated cell was characterize...

α-1,3-Glucan is present as the outermost layer of the cell wall in the pathogenic yeastlike (Y) form of Paracoccidioides brasiliensis. Based on experimental evidence, this polysaccharide has been proposed as a fungal virulence factor. To degrade α-1,3-glucan and allow remodeling of the cell wall, α-1,3-glucanase is required. Therefore, the study of this enzyme, its encoding gene, and regulatory...

The autolytic release of fl-fructofuranosidase (EC 3.2.1.26) and acid phosphatase (EC 3.1.3.2) from the cell wall of yeast was studied under different organic solvents and with a variety of pII and temperature conditions. /3Fructofuranoeidase (but not acid phosphatase) was found to be a convenient marker for studying changes in the wall structure. The ethyl acetate-treated cell was characterize...

A rapid filtration test method that predicts malt efficiency has been developed in this research. This an advantage over existing beer tests, as it can be easily operated and there is no need to brew. Six kinds of malts with distinct performance were selected, the was determined by measuring turbidity their clarified wort through simple simulation mashing. The results indicate filtering ranking...

Lysobacter enzymogenes strain N4-7 produces multiple biochemically distinct extracellular -1,3-glucanase activities. The gluA, gluB, and gluC genes, encoding enzymes with -1,3-glucanase activity, were identified by a reverse-genetics approach following internal amino acid sequence determination of -1,3-glucanase-active proteins partially purified from culture filtrates of strain N4-7. Analysis ...

Clostridium thermocellum produces one major beta-1,3-glucanase. Genomic DNA fragments containing the gene were cloned from two strains, DSM1237(T) (6848 bp) and F7 (9766 bp). Overlapping sequences were 99.9 % identical. The nucleotide sequences contained reading frames for a putative transposase, endo-beta-1,3-1,4-glucanase CelC, a putative transcription regulator of the LacI type, beta-1,3-glu...

Mycobacterium tuberculosis (Mtb) remains a leading cause of morbidity and mortality worldwide, as two billion people are latently infected with Mtb. To address Mtb drug resistance and the limitations of current vaccines, the characteristics of candidate Mtb vaccines need to be explored. Here, we report the three-dimensional structure of Rv0315 at 1.70 Å resolution, a novel immunostimulatory ant...