The effect of glucosamine on phenotypic mixing between vesicular stomatitis virus (VSV) and avian sarcoma virus (ASV) was studied. Phenotypic mixing decreased with increase in glucosamine concentration, and, in the presence of 20 mM glucosamine, was no longer detectable. In the presence of 20 mM glucosamine, cells still produced 10(2)--10(3) focus forming units (FFU) of ASV and 10(6) plaque for...

Cell suspensions of Micrococcus sodonensis (ATCC 11880) secrete 7 to 10 individual proteins including an alkaline phosphatase, a nuclease, and a protease. The appearance of enzyme activities in the extracellular medium is dependent on the co-secretion of at least one of several polysaccharides also elaborated by these cells. This conclusion is based on the following observations: (a) bacitracin...

In the present study, the effect of glucosamine administration (1.5 or 3 g/day) on cartilage and bone metabolism was investigated in bicycle racers, using cartilage‑ and bone‑specific biomarkers, including C‑terminal cross‑linked telopeptides of type II collagen (CTX‑II), C‑terminal propeptides of type II procollagen (CPII), N‑terminal telopeptide...

Osteoarthritis (OA) is a progressive joint disease, that occurs frequently in the aging population and is a major cause of disability worldwide. Both glucosamine and chondroitin are biologically active molecules that are substrates for proteoglycan, an essential component of the cartilage matrix. Evidence supports the use of glucosamine and chondroitin as symptomatic slow-acting drugs for osteo...

The sugar nucleotide analogue UDP-glucosamine was found to function as a sugar donor in microsomal preparations of both chick-embryo cells and rat liver, yielding dolichyl monophosphate glucosamine (Dol-P-GlcN). This was characterized by t.l.c. and retention by DEAE-cellulose. Glucosamine was the only water-soluble product released on mild acid hydrolysis. Dol-P-GlcN did not serve as substrate ...

Chitin is a polysaccharide component of the inner cell wall fungi that has been used to estimate fungal invasion in plant products. However, its detection major pathogenic species not investigated. The present study aimed determine chitin contents order evaluate diagnostic potential as biomarker for infections. High performance liquid chromatography (HPLC) was measure content. Pure acid hydroly...

BACKGROUND There is concern that shrimp-allergic individuals may react to glucosamine-containing products as shrimp shells are a major source of glucosamine used for human consumption. OBJECTIVE The purpose of this study was to determine whether shrimp-allergic individuals can tolerate therapeutic doses of glucosamine. METHODS Subjects with a history of shrimp allergy were recruited and tes...