Conventional enzyme-based continuous glucose sensors in interstitial fluid usually rely on dissolved oxygen as the electron-transfer mediator to bring electrons from oxidase electrode while generating hydrogen peroxide. This may lead several problems. First, sensor provide biased detection results owing fluctuation of fluid. Second, polymer coatings that regulate glucose/oxygen ratio can affect...

The measurement of fluorescence quenching activity of [Ru(L)3] with 4,7-diphenyl1,10 phenanthroline (dip) on silica gel and embedded in polystyrene polymers for the determination of dissolved oxygen using fiber optic probe is described here. The system is extended by immobilization of glucose oxidaze (GOD) for the determination of glucose. The objective of the work is to conquer the leaching an...

We present a MEMS sensor aiming to enable continuous monitoring of glucose levels in diabetes patients. The device features a magnetically-driven vibrating microcantilever, which is situated in a microchamber and separated from the environment by a semi-permeable membrane. Glucose sensing is based on affinity binding principles using a solution of dextran concanavalin-A (Con A) as the sensing f...

BACKGROUND Standard care for infants on extracorporeal life support (ECLS) relies on intermittent measurement of blood glucose (BG); however, this can lead to significant changes in BG that go unrecognized for several hours. The present study was designed to assess performance and clinical applicability of a subcutaneous glucose sensor technology modified for use as a blood-contacting sensor wi...

The changes in plasma glucose concentration and in interstitial glucose concentration, determined with a miniaturized subcutaneous glucose sensor, were investigated in anesthetized nondiabetic rats. Interstitial glucose was estimated through two different calibration procedures. First, after a glucose load, the magnitude of the increase in interstitial glucose, estimated through a one-point cal...

BACKGROUND Predictive low glucose management (PLGM) may help prevent hypoglycemia by stopping insulin pump delivery based on predicted sensor glucose values. MATERIALS AND METHODS Hypoglycemic challenges were simulated using the Food and Drug Administration-accepted glucose simulator with 100 virtual patients. PLGM was then tested with a system composed of a Paradigm(®) insulin pump (Medtroni...

AIMS/INTRODUCTION The present study aimed to investigate the performance of a new real-time continuous glucose monitoring system. MATERIALS AND METHODS Interstitial glucose levels were monitored for 7 days in 63 patients with type 1 or type 2 diabetes using the Medtrum A6 TouchCare® CGM System. Venous blood was collected on a randomized day of the wear period. Plasma glucose levels were measu...

We have combined a microfluidic network and developed a miniature glucose sensor to measure glucose consumption of single cardiomyocytes in a confined extracellular space. The thin film glucose sensor was integrated into a 360 picoliter extracellular space. We used Pt thin film electrodes (20x100 μm) as a counter electrode, and a needle type reference electrode (0.45 mm diam) placed in the cell...

Glucose concentration may be determined enzymatically by a glucose-oxidase procedure in which the amount of oxygen depletion from a solution saturated with oxygen is observed. The difference between the initial and final oxygen levels as detected by a polarographic oxygen analyzer is a measure of glucose concentration. Blood is sampled continuously by means of a double-lumen catheter inserted i...

A novel poly(vinyl alcohol) grafting 4-vinylpyridine self-gelatinizable copolymer was adapted to immobilize glucose oxidase. The reduction of hydrogen peroxide (H2O2) was detected at a Prussian Blue (PB) modified graphite electrode. A stable and sensitive glucose amperometric biosensor is described. The copolymer is a good biocompatible polymer in which the glucose oxidase retains high activity...