BACKGROUND Most β-glucosidases reported are sensitive to the end product (glucose), making it the rate limiting component of cellulase for efficient degradation of cellulose through enzymatic route. Thus, there are ongoing interests in searching for glucose-tolerant β-glucosidases, which are still active at high glucose concentration. Although many β-glucosidases with different glucose-toleranc...

BACKGROUND Glycosylation of flavonoids is a promising approach to improve the pharmacokinetic properties and biological activities of flavonoids. Recently, many efforts such as enzymatic biocatalysis and the engineered Escherichia coli biotransformation have increased the production of flavonoid glucosides. However, the low yield of flavonoid glucosides can not meet the increasing demand for hu...

Principle guided design of glycan processing enzyme inhibitors involves embedding aromatic groups onto charge and shape mimics. Intramolecular azide-alkyne cycloaddition was used as a simple and versatile strategy for the synthesis of novel condensed bicyclic triazoles from carbohydrate derived Perlin aldehydes. These newly synthesised molecules were evaluated for glycosidase inhibition against...

16 Cellulose, the major constituent of all plant materials and the most abundant 17 organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected 18 by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of 19 hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases) and 20 β-glucosidases acting in a synergistic manner. In bi...