UNLABELLED Background The number of CD34(+) cells mobilized from bone marrow to peripheral blood after administration of granulocyte colony-stimulating factor varies greatly among healthy donors. This fact might be explained, at least in part, by constitutional differences in genes involved in the interactions tethering CD34(+) cells to the bone marrow. DESIGN AND METHODS We analyzed genetic ...

The assessment of granulocyte chemotaxis is complicated by the difficulty of precisely reproducing results in serial estimations and deciding on the best end point which would reflect most accurately the degree of travel taken by the cells under observation. The methods in use are generally based on the Boyden chamber, following this, we have further developed the principle of the "raft" techni...

Disseminated intravascular coagulation (DIC) is a recurrent complication of sepsis. Since DIC not only promotes organ dysfunction but also represents strong prognostic factor, it important to diagnose as early possible. When activated, fibrinolysis inhibited, blood thinners are consumed, and condition created that clotting, making more difficult for the body remove fibrin or prevent from being ...

The performance characteristics of the XE-2100 (Sysmex, Kobe, Japan) automated immature granulocyte (IG) count were studied. The automated IG count was compared with the manual morphology count and with a proposed reference flow cytometric count. The comparison data were analyzed by both least-squares and Passing-Bablok regression analysis. Long-term imprecision using preserved blood quality co...

In the background section of their abstract, Woei-Cherng Shyu and associates state that because granulocyte colony-stimulating factor (G-CSF) has anti-inflammatory and neuroprotective properties and the capacity to mobilize stem cells, it has the potential to be used in treatment of stroke. However, there are limited data about the effects of G-CSF on hemostasis. It has been suggested that G-CS...

In this study granulocyte-macrophage colony stimulating factor (CSF) was prepared from rabbit and guinea pig lung tissues. The biological activity of these CSFs was measured on semisolid agar culture and compared with those of mouse and rat. Weight of animals, incubation time and cross-reactivity of CSFs with different bone marrow sources were also investigated. The results showed that both...

The effect on granulopoiesis of 6-mercaptopurine alone, and in combination with allopurinol, was studied in mice using an in vitro colony-forming assay for granulocytic progenitor cells. The combination of 6-mercaptopurine and allopurinol pro duced significantly greater suppression of de novo granulo poiesis than did 6-mercaptopurine alone. Allopurinol in very high doses was found to be myelosu...