Short cationic oligomers, including arginine-rich peptides and analogous beta-amino acid oligomers ("beta-peptides"), can enter the cytoplasm and nucleus of a living cell from the extracellular medium. It seems increasingly clear that multiple entry pathways are possible, depending upon the structure of the guanidinium-rich molecule, the type of cell, and other factors. We have previously shown...

Nucleic acid extraction is an attractive alternative to labor-intensive manual methods. In this report, a few methods of total RNA Extraction from Edible mushroom (Agaricus bisporus) compared. This methods consist AGPC(Acid Guanidinium-Phenol-Chloroform), The method according by Lithium chloride and RNx total RNA isolation Kit (CinnaGen). Best method selected for the preparation of high quality...

The polypeptide chain location of active site serine and carbohydrate in the multiple forms of thrombin present in Parke-Davis thrombin have been investigated by means of [3H]diisopropylfluorophosphate incorporation and carbohydrate staining techniques. Both the sodium dodecyl sulfate electrophoretic technique and gel filtration in 6 M guanidinium chloride have been applied to the resolution of...

Human platelet proteoglycan (P.PG) was prepared from a 4 M-guanidinium chloride platelet extract in the presence of proteinase inhibitors. The purification procedure included CsCl-density-gradient centrifugation, DEAE-Sepharose CL-6B ion-exchange chromatography and f.p.l.c. on a Mono Q HR 5/5 column. P.PG was recovered as a polydisperse molecule, but the protein core appeared to be at least 90%...

The selectivity of sodium channels in squid axon membranes was investigated with widely varying concentrations of internal ions. The selectivity ratio, PNa/PK, determined from reversal potentials decreases from 12.8 to 5.7 to 3.5 as the concentration of internal potassium is reduced from 530 to 180 to 50 mM, respectively. The internal KF perfusion medium can be diluted by tetramethylammonium (T...

The effects of urea and of guanidinium chloride on binding constants in water for 6-(4-tert-butylanilino)-naphthalene-2-sulfonate and of bis(p-tert-butylphenyl) phosphate binding to beta-cyclodextrin and to N,N'-bis(6-beta-cyclo-dextrinyl)imidazolium ion have been determined. Their effects on the water solubility of p-tert-butylbenzyl alcohol and p-methylbenzyl alcohol have also been examined. ...

Hirudin is a 65-amino acid polypeptide with three disulfide linkages. It is stable under extreme pH (1.4712.9), high temperature (95 “C), and in the presence of denaturants (6 M guanidinium chloride or 8 M urea). The thrombin inhibitory activity of hirudin remains unaffected even after cleavage of an internal peptide bond ( L y ~ ~ ‘ A s n ~ ~ ) . One condition which effectively and irreversibl...

A protocol is presented for the quantitative conversion of cysteine and cystine residues in proteins to residues of Scyanocysteine, using the reagent 2-nitro-S-thiocyanobenzoic acid (DEGANI, Y., AND PATCHORNIK, A. (1971) J. Org. Chem. 36, 2727). Cleavage of the amino peptide bond of the Scyanocysteine residue is obtained upon exposure to 6 M guanidinium chloride-O.1 M sodium borate, pH 9.0, at ...

Very lysine-rich histones were extracted by aqueous trichloracetic acid from calf thymus, from the thymus, mammary gland, and liver of rabbits, and from chicken livers. The elution profiles of these histones, obtained by chromatography on Amberlite IRC-50 with a shallow guanidinium chloride gradient, revealed that the very lysine-rich histone complement varied from one animal species to another...