Background Toxic effects of anti-cancer and other drugs on the normal tissues could be reduced by the herbal plants and their fractions. This study investigated the protective effect of thymoquinone (TQ) as a fraction of Nigella sativa on methotrexate (MTX)- induced germ cell apoptosis in male mice. MaterialsAndMethods In this experimental study, thirty male Balb/c mice were divided randomly in...

Various investigators have reported changes in the concentration of the free amino acids in blood plasma and tissues of guinea-pigs in acute vitamin C deficiency. Christensen & Lynch (1948) found in guinea-pigs deficient in ascorbic acid a large decrease in the glycine and glutamine contents of skeletal muscle and in the glutamine content of the liver. Levels of amino acids other than glycine a...

Objective(s): For determining the mechanism of anti-asthmatic effect of thymoquinone, this investigation evaluated the effect of thymoquinone in the presence of selective A2A and A2B adenosine receptor antagonists (ZM241385 and MRS1706, respectively). Materials and Methods: Seventy guinea pigs were randomly divided to 7 groups; control (C), sensitized with ovalbumin (S), sensitized groups pretr...

Two hepatic cytoplasmic protein fractions, designated Y and Z, which bind sulfobromophthalein (BSP), bilirubin, and other organic anions, have been separated by G75 Sephadex gel filtration. The physiologic role of these protein fractions has been investigated. They are present in the 110,000 g supernatant fraction from the livers of all the species tested (rats, mice, guinea pigs, Rhesus monkey...

OBJECTIVE To assess the validity of the guinea pig as a model for congenital cytomegalovirus (CMV) infection by comparing the effectiveness of detecting the virus by real-time polymerase chain reaction (PCR) in blood, urine, and saliva. DESIGN Case-control study. SETTING Academic research. SUBJECTS Eleven pregnant Hartley guinea pigs. MAIN OUTCOME MEASURES Blood, urine, and saliva sampl...

Hemagglutination is a specific and sensitive technique for investigating the purity of lipoproteins and the immunologic relationships between low density lipoprotein fractions. The S(f) 10-400 and S(f) 3-9 lipoprotein fractions, isolated from human serum by dextran sulfate-density gradient centrifugation procedure and repurified by centrifugation appeared to contain only lipoprotein antigens si...

Background and objectives: Nigella sativa seeds possess anti-inflammatory, antioxidant, tranquillizer, chemoprotective and antineoplastic effects. They seeds are important nutritional flavoring agents and natural remedies for many aliments. We have investigated the protective effects of N. sativa seed fatty acids against doxorubicin. Methods: ...