The yeast Hansenula wingei is a favourable organism for study of the physiology of the conjugation process (cell fusion). Microscopic observations on fusion are presented which reveal that the mating cells in contact fuse by a softening of the cell wall, followed by formation of a conjugation tube, dissolution of the cross-walls between them, and formation of a new bud a t the point of juncture...

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We have isolated the Hansenula polymorpha CPY gene encoding carboxypeptidase Y (Hp-CPY). The deduced amino acid sequence revealed that Hp-CPY consists of 541 amino acids and has a calculated Mr of 60,793. The protein is highly similar to Saccharomyces cerevisiae CPY (61.8% identity). At the N-terminus of Hp-CPY signals for the entry into the secretory pathway and subsequent sorting to the vacuo...

Brock, Thomas D. (Indiana University, Bloomington). Biochemical and cellular changes occurring during conjugation in Hansenula wingei. J. Bacteriol. 90:1019-1025. 1954.-A technique has been devised for deagglutinating mixed populations of conjugating cells so as to be able to visualize microscopically early stages of the conjugation process. A cell can form a conjugation tube only when in conta...

Peroxisomes are essential organelles in many eukaryotes. Until recently, the main focus of the investigations concerning this important organelle was to understand the biogenesis of the peroxisome (induction, proliferation and matrix protein import). However, when peroxisomes become redundant they are quickly degraded by highly selective processes known as pexophagy. The first molecular studies...

The recent elucidation of the enzyme systems involved in nitrate reduction in Neurospora crassa (Evans and Nason, 1952; Nason and Evans, 1953); Bacillus pumilis (Taniguchi et al., 1953); Escherichia coli (Taniguchi et al., 1956; Nicholas and Nason, 1955) and higher plants (Evans and Nason, 1953), has renewed interest in the general problem of nitrate reduction and nitrate assimilation in microo...

The yeast cell wall is constantly remodeled to enable cell growth and division. In this study, we describe a novel type of cell wall modification. We report that the drug amiodarone induces rapid channel formation within the cell wall of the yeast Hansenula polymorpha. Light microscopy shows that shortly after adding amiodarone, spherical structures, which can be stained with DNA binding dyes, ...

The yeast Cyberlindnera fabianii is used in wastewater treatment, fermentation of alcoholic beverages, and has caused blood infections. To assist in the accurate identification of this species, and to determine the genetic basis for properties involved in fermentation and water treatment, we sequenced and annotated the genome of C. fabianii (YJS4271).