Serum samples from 356 HBsAg positive asymptomatic carriers, which were titrated by reverse passive hemagglutination, were analysed for the presence of HBV-DNA, HBsAg and IgM anti-HBc. The samples were divided in three classes, according to the titers of HBsAg and IgM anti-HBc and the distribution of HBV-DNA and HBsAg among these classes was studied. In the high titer class of HBsAg, 65% of sam...

Tobacco plants were genetically transformed with the gene encoding hepatitis B surface antigen (HBsAg) linked to a nominally constitutive promoter. Enzyme-linked immunoassays using a monoclonal antibody directed against human serum-derived HBsAg revealed the presence of HBsAg in extracts of transformed leaves at levels that correlated with mRNA abundance. This suggests that there were no major ...

Abstract Introduction The presence of hepatitis B virus (HBV) surface antigen (HBsAg) in serum is the hallmark of HBV infection, and qualitative tests for its detection have been used since 1969. Recently, HBsAg quantification has been applied for monitoring chronic HBV infection during its natural history as well as for the prediction of the response to treatment. As HBsAg titres are becoming ...

Hepatitis B virus (HBV) is considered as a global health concern and hepatitis B surface antigen (HBsAg) is the most immunogenic protein of HBV. The purpose of this study was to evaluate the expression of HBsAg on the cell surface of human embryonic kidney cell line (HEK293T). After transformation of expression vector pcDNA/HBsAg to E.coli TOP10F', plasmid was extracted and digested with BglII....

Quantitative hepatitis B surface antigen (HBsAg) is a valuable tool in hepatitis B virus (HBV) disease diagnosis and management for evaluating the effectiveness of antiviral therapy. The aim of the current research was to compare the performances of the Elecsys HBsAg II and Abbott Architect HBsAg assays in chronic hepatitis B (CHB) patients. Between May 2014 and December 2014, 72 CHBs were test...

The ability of human semen and saliva to transmit hepatitis B virus (HBV) by parenteral and nonparenteral routes was studied. Semen, donated by a hepatitis B surface antigen (HBsAg)- and hepatitis B e antigen (HBeAg)-positive carrier, was administered to one gibbon by subcutaneous inoculation and to another by intravaginal instillation. Both developed HBsAg, followed by the development of antib...

BACKGROUND/AIMS Decay of hepatitis B surface antigen (HBsAg) titers has previously been shown to be predictive of a virologic response (VR), especially during peginterferon-alpha therapy. However, the role of HBsAg levels in predicting a VR to nucleos(t)ide analog therapy has not yet been established. In this study we sought to determine whether the VR can be predicted from HBsAg titers in nucl...

The prevalence of Hepatitis B Virus (HBV) infection in inflammatory bowel disease (IBD) has been reported differently across the world. The present study retrospectively included 675 consecutive IBD patients (449 Crohn's disease, CD and 226 ulcerative colitis,UC) from July 2006 to July 2012. The rates of HBV infection (HBsAg-positive) were 13.6%, 16.8% and 13.8% in patients with CD, UC, and gen...

Complexes between hepatitis B surface antigen (HBsAg) and immunoglobulin M (IgM) have been detected in acute type B hepatitis. Sequential serum testing for the presence of these complexes has been shown to be the best method for predicting disease chronicity. The presence of HBsAg/IgM complexes was investigated using an enzyme-linked immunosorbent assay with selected sera from Senegal. The thre...

INTRODUCTION The aim of this study was to determine hepatitis B surface antigen (HBsAg) seroclearance rate among patients treated with lamivudine at a specialized tertiary care referral hospital in Tehran, Iran. METHODS All patients on lamivudine (biovudin®) therapy at a dose of 100 mg/day, who showed seroclearnace between March 2001 and September 2011 were recruited. The main evaluation para...