Prostacyclin release from endothelial cells in culture appears increased by the presence of plasma, but occurrence of a similar phenomenon in intact vessels has not been established. In the present investigation release of 6-keto-PGF quantitated from &i the stable breakdown product of prostacyclin, was ne veins perfused ex vivo for 15 minute periods, using three different perfusates: 1) Hank’s ...

A systematic review and meta-analysis of observational studies were conducted to quantify the association between sickle cell disease in pregnancy and adverse maternal and perinatal outcomes. Data sources (Medline, Embase, Maternity and Infant care, Cochrane, Web of Science, Popline) were searched for publications to June 2014. Eligibility criteria included observational studies reporting mater...

OBJECTIVE There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ) as a storage medium for retaining avuls...

Little data exist for the mechanical properties of individual irreversible or reversible sickle cells (ISC and RSC, respectively), nor is the process of ISC formation well understood. For oxygenated ISC and density-fractionated RSC, we have used micropipette techniques to measure cell surface area (SA) and volume (V), membrane shear elastic modulus (mu), time constant for viscoelastic shape rec...

Abstract Researches proving methods for nano-encapsulation of neonatal porcine islet-like cell clusters (NPCCs) using polymersomes (PSomes) formed polymers polyethylene glycol-block-poly lactide. Herein, our studies present efficient procedure with minimal damage and loss NPCCs.We used N-hydroxysuccinimide (NHS) on the N-terminal PSomes to induce binding amine groups in extracellular matrix sur...

BACKGROUND Adrenal, thyroid, and parathyroid gland hormonal changes are recognized in children with homozygous (HbSS) sickle-cell anemia (SCA), but are not clear in adult patients with SCA. AIM To assess the metabolic and endocrine abnormalities in adult patients with SCA and evaluate left ventricular (LV) systolic and diastolic functions compared with patients with no SCA and further study t...

Sickle cell anemia was first described by Herrick in 1910. Pioneering studies by Pauling et al. established that Sickle Cell Disease (SCD) results from a defect in the hemoglobin molecule. 1 The sickle mutation was characterized several years later by Ingram et al. as a glutamine-to valine substitution at the sixth residue of the beta globin polypeptide. Homozygosity for the sickle mutation (i....

After its discovery as a genetically inherited hemoglobinopathy one hundred years ago, finding a widely available cure for sickle cell anemia (SCA, HbSS) still remains a challenge and the disease requires multi-disciplinary theranostic approaches (Menaa et al., 2013; Menaa, 2014). Indeed, more effort and resources to promptly find alternative therapeutics and/or adjuvants (e.g. nutraceutics) to...

Background: Deoxygenation of sickle erythrocytes activates a cation permeability of unknown molecular identity (Psickle), leading to elevated intracellular [Ca] ([Ca]i) and subsequent activation of KCa 3.1. The resulting erythrocyte volume decrease elevates intracellular hemoglobin S (HbSS) concentration, accelerates deoxygenation-induced HbSS polymerization, and increases the likelihood of cel...