background: light chain (lc) and heavy chain carboxyterminal subdomain (hcc) fragments are the most important parts of tetanus neurotoxin (tent) which play key roles in toxicity and binding of tent, respectively. in the present study, these two fragments were cloned and expressed in a prokaryotic system and their identity was confirmed using anti-tent specific polyclonal and monoclonal antibodi...

Table 1. Glomerular Deposition Diseases and Fibrillary Glomerulopathies A: Kidney (glomerular deposition diseases) 1. AL AH amyloidosis 2. MIDD (non-amyloid monoclonal immunoglobulin deposition disease) a. LCDD (light chain deposition disease b. LHCDD (light and heavy chain deposition disease) c. HCDD (Heavy chain deposition disease) 3. Light chain cast nephropathy 4. Fibrillary glomerulonephri...

Previous work indicates that the heavy chain of tetanus toxin is responsible for the binding of the toxin to the neuronal membrane and its subsequent internalization. In the present study, the light chain of tetanus toxin mimicked the holotoxin in inhibiting Ca2+-dependent secretion of [3H]norepinephrine from digitonin-permeabilized adrenal chromaffin cells. Preincubation of tetanus toxin with ...

Two variants in immunoglobulin heavy chain production, derived from the MPC 11 mouse myeloma cell line, make short heavy (H) chains with identical precise deletions of the CH3 domain. The CH3 domain is expressed in the H chain mRNA from both variants. Although in vitro translation of this mRNA produces one H chain species, deleted heavy chains are secreted as heavy-light (HL) and H2L2 moieties ...

A series of 15 patients with hairy-cell leukemia was studied for SI9. Mononuclear cells isolated from peripheral blood (15), spleen (6), bone marrow (2), and lymph node (1) were tested for SIg by a sensitive rosette method, and extensive precautions were taken to avoid the various problems causing nonintrinsic Ig binding and to ensure the specificity of the antisera. Slg of restricted light cha...

Human plasma kallikrein consists of an N-terminal heavy chain of molecular weight (mol wt) 52,000, linked by disulfide bonds to two light chain variants (mol wt 36,000 or 33,000). Although the active catalytic site of kallikrein resides on the C-terminal light chain, the role of the N-terminal heavy chain is less clear. We therefore studied an enzyme designated beta-kallikrein, containing a sin...

We have described a mouse T cell alloantigen, Tsud, 1 which may be an allotypic or idiotypic determinant on a T cell receptor (1-3). Tsu d is associated with the T cell receptor for idiotype, apparent ly on suppressor T cells or their precursors (2). The determinant is defined by antisera made in B A L B / c mice against T cell blasts from allotype congenic C.AL-20 mice and can be detected by i...

Immune recognition by cytotoxic effector T cells requires participation of the CD8 and major histocompatibility complex class I antigens. We found that the CD8 molecule is noncovalently associated with the HLA class I heavy chain on the surface of human T cells activated by Con A. Accordingly, anti-CD8 monoclonal antibodies precipitated a heterodimer containing polypeptides of 32 and 43 kDa fro...

Two regions on the clathrin heavy chain that are involved in triskelion interactions during assembly have been localized on the triskelion structure. These regions were previously identified with anti-heavy chain monoclonal antibodies X19 and X35, which disrupt clathrin assembly (Blank, G. S., and F. M. Brodsky, 1986, EMBO (Eur. Mol. Biol. Organ.) J., 5:2087-2095). Antibody-binding sites were d...

Secretory immunoglobulin (Ig) A is a decameric Ig composed of four alpha-heavy chains, four light chains, a joining (J) chain, and a secretory component (SC). The heavy and light chains form two tetrameric Ig molecules that are joined by the J chain and associate with the SC. Expression of a secretory monoclonal antibody in tobacco (Nicotiana tabacum) has been described: this molecule (secretor...