This paper handles the medical waste disposal facility location problem in Istanbul by using fuzzy TOPSIS (FETOPSIS) to select the adequate place between some candidate points that are obtained from the Ishii’s undesirable facility location algorithm. Medical waste must be disposed without damaging environment and human health, complying with new regulations. The aim is to provide an alternativ...

A method for fluorescent labeling of PCR products has been developed. This method consists in a two-step procedure in which a first exponential classical PCR is followed by a "linear amplification". This second step relies on incorporation of fluorescent dNTP (dUTP or dCTP) in order to label the product on only one strand. The products can be applied without prior purification directly to a gel...

Background: Gingival recessions require treatment for many reasons – impaired aesthetic appearance, root sensitivity, cervical caries or abrasion. Two surgical techniques have been described that use free gingival graft for root coverage. The technique proposed by Bernimoullin et al. involves two surgical steps. The first step consists of creating attached gingiva by means of free gingival graf...

1242 CLINICALCHEMISTRY, Vol.31, No.7, 1985 Clin Chem 29, 355-357 (1983). 2. Viberti G, Keen H. The patterns of proteinuria in diabetes mellitus: Relevance to pathogenesis and prevention of diabetic nephropathy. Diabetes 33,686-692(1984). 3. Garner RC, Walpole AL, Rose FL. Testing of some benzidine analogues for microsomal activation to bacterial mutagens. Cancer Lett 1, 39-42 (1975). 4. Miles O...

We propose a new method for soft spatial segmentation of matrix assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) data which is based on probabilistic clustering with subsequent smoothing. Clustering of spectra is done with the Latent Dirichlet Allocation (LDA) model. Then, clustering results are smoothed with a Markov random field (MRF) resulting in a soft probabilisti...

The liver, an organ with an exceptional regeneration capacity, carries out a wide range of functions, such as detoxification, metabolism and homeostasis. As such, hepatocytes are an important model for a large variety of research questions. In particular, the use of human hepatocytes is especially important in the fields of pharmacokinetics, toxicology, liver regeneration and translational rese...

In this paper we discuss the differences between the average marginal effect and the marginal effect of the average individual in sample selection models, estimated by Heckman’s two step procedure. We show that the bias that emerges as a consequence of interchanging them, could be very significant, even in the limit. We suggest a computationally cheap approximation method, which corrects the bi...

For situations where the number of tested hypotheses is increasingly large, the power to detect statistically significant multiple treatment effects decreases. As is the case with microarray technology, often researchers are interested in identifying differentially expressed genes for more than two types of cells or treatments. A two-step procedure is proposed for the purpose of increasing powe...

Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, beta-spectrin, alpha-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to res...

A microwave-assisted, one-pot, two-step protocol was developed for the construction of polysubstituted 2-aminoimidazoles. This process involves the sequential formation of imidazo[1,2-a]pyrimidinium salts from readily available 2-aminopyrimidines and alpha-bromocarbonyl compounds, followed by opening of the pyrimidine ring with hydrazine. [reaction: see text]