The internalization of the N-terminal catalytic domain of Bordetella pertussis adenylate cyclase toxin (ACT) across the cytoplasmic membrane has been considered to occur independently from protein-protein interactions which can lead to oligomerization required for hemolytic activity by its C-terminal hemolysin domain. Here we report that when added in excess, this hemolysin domain stimulates th...

Here we determined the role of various genomic islands in E. coli K1 interactions with phagocytic A. castellanii and nonphagocytic brain microvascular endothelial cells. The findings revealed that the genomic islands deletion mutants of RS218 related to toxins (peptide toxin, α -hemolysin), adhesins (P fimbriae, F17-like fimbriae, nonfimbrial adhesins, Hek, and hemagglutinin), protein secretion...

staphylococci isolated from 98 mastitic cow’s milk samples were analyzed for the production of alpha,beta and delta-hemolysin on sheep, bovine and horse red blood cells and in vitro susceptibility to several antimicrobial agents. out of a total of 18 isolated strains, 100% demonstrated hemolytic activity either alone or in combined forms. among 8 isolates of s. aureus 3 isolates produced α/β an...

The iron uptake systems of Plesiomonas shigelloides strains were determined. Siderophore production was not detected by chemical or biological assays, and the strains tested were unable to use enterobactin, aerobactin, or vibriobactin for growth in low-iron media. Both hemin and hemoglobin supported full growth of the bacteria in media lacking other iron sources, but neither transferrin nor lac...

Certa in propert ies of a hemolysin associated with mumps virus have been described in a previous repor t (1). The labile na ture of this hemolyt ic principle was indicated b y its inact iva t ion b y hea t and b y its deter iorat ion during storage under a va r ie ty of conditons. In order to faci l i tate fur ther invest igat ion of this v i ra l hemolysin, i t was necessary to determine the ...

Brachyspira hyodysenteriae is the primary cause of swine dysentery, which is responsible for major economic losses to the pig industry worldwide. The hemolytic activity of 10 B. hyodysenteriae strains isolated from stools of pigs with mild to mucohemorrhagic diarrhea was compared and seven hemolysis associated genes were sequenced. Hemolysis induced by these strains varied from strong to near a...

By CARL F. HINz, JR. T HE HEMOLYSIN of the Donath-Landsteiner reaction, responsible for [)arOxysmal cold hemoglobinuria, has not been characterized physicochemically. Other erythrocyte agglutinins and hemolysins have been identified in both the 75 and 195 fractions of y-globulin,1 and certain other of the cold antibodies have been identified with the 195 globulins.2 Further, the Donath-Landstei...

Heat-stable hemolysin produced by the cellophane-agar plate method consisted of two major acidic glycolipids. One was composed of 2 mol of rhamnose and 2 mol of beta-hydroxydecanoic acid, and the other was composed of 1 mol of rhamnose and 2 mol of beta-hydroxydecanoic acid. The hemolysis-producing moiety of the hemolysin was shown to be the dimer of beta-hydroxydecanoic acid contained in the g...