Age-related macular degeneration is a multifactorial disease affecting the posterior segment of eye and characterized by aberrant nascent blood vessels that leak fluid. It ends with vision loss. In present study, artemisinin which poorly water-soluble has potent anti-angiogenic anti-inflammatory properties was formulated into nanomicelles for its ocular application activity using CAM assay. Art...

Introduction. Secreted metabolites with enzymatic or toxic properties are important for effecting of Candida spp pathogenicity. The most clinically significant species is C. albicans. However, invasive infections associated auris have a great significance.
 aim the investigation comparison some and biocidal activity those albicans.
 Materials methods. albicans strains were used in stu...

BACKGROUND hGlyrichin is a novel human antimicrobial peptide rich in glycine. The previous study of known human antimicrobial peptides indicated that in an eligible range, the greater corresponding antibacterial activity was consisted with the shorter peptide sequence. FINDINGS Two peptides named pCM19 and pCM12 were synthesized and the antibacterial activity assay results showed that these p...

Twenty-four patients with aplastic anemia or acute leukemia were treated by marrow grafts from HLA-identical donors after conditioning with high doses of cyclophosphamide and/or total body irradiation. They were studied between 4 and 63 mo (median 14.2) after transplantation. Seventeen patients had chronic graft-versus-host disease (C-GVHD) and 7 were healthy. They were studied for defects in t...

Aim. In this study we focused on the search of fibrinogen-targeted proteases in venom Vipera lebetina. Methods. Fractionation was performed using FPLC chromatographic system Acta Prime Q Sepharose. Analysis protein mixtures SDS-PAGE. Аction blood coagulation analyzed APTT assay [2]. Proteolytic action fibrinogen and identification components with fibrinolytic activity electrophoresis mixture so...

Relating thermodynamic parameters to structural and biochemical data allows a better understanding of substrate binding and its contribution to catalysis. The analysis of the binding of carbohydrates to proteins or enzymes is a special challenge because of the multiple interactions and forces involved. Isothermal titration calorimetry (ITC) provides a direct measure of binding enthalpy (DeltaHa...

The measurement of total haemolytic complement is of diagnostic value in the rheumatic diseases. A method which is easier to perform than that of Kabat and Mayer (Experimental Immunochemistry, 2nd ed., Thomas, Springfield, Ill.) is described. A unit based on the mean value of the complement activities of normal sera is proposed to replace CH50 units. Other steps by which standardization of the ...

The total haemolytic complement (CH50), the complement components C3 and C4, the complement breakdown product C3d, alternative pathway activation and transferrin, were measured before, during and after cardiopulmonary bypass. As expected, CH50 decreased after heparinization, remained low during bypass and decreased further up to 8 h after bypass. C3 and C4 decreased significantly during bypass,...

This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affinity data by isothermal titration calorimetry (ITC) and by differential scanning fluorimetry (DSF) ...