Comparative bacterial genomics shows that even different isolates of the same bacterial species can vary significantly in gene content. An effective means to survey differences across whole genomes would be highly advantageous for understanding this variation. Here we show that suppression subtractive hybridization (SSH) provides high, representative coverage of regions that differ between simi...

The effect of living hemolytic streptococci on ascites tumor cells (Sarcoma 37 and Krebs-2 carcinoma) was studied in vitro by phase microscopy and in vivo by implanta tion into mice. The effects produced by Streptococcus pyogenes strains were compared with those of Serraiia marcescena, Sareina lutea, and Streptococcuafuecalis. Three of the eight streptococcal strains were highly effective again...

The threat of pandemic and highly pathogenic influenza viruses currently represents a top global public health problem. Majority of deaths in patients with severe influenza infections are due to complications resulting from impaired gas-exchange, pulmonary edema and alveolar-capillary damage, which are characteristics of acute respiratory distress syndrome (ARDS) [1,2]. Although vaccination is ...

We describe a novel cloning method termed SLiCE (Seamless Ligation Cloning Extract) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of traditional cloning methods, facilitates seamless cloning by recombining short end homologies (≥15 bp) ...

Comparative bacterial genomics shows that even different isolates of the same bacterial species can vary significantly in gene content. An effective means to survey differences across whole genomes would be highly advantageous for understanding this variation. Here we show that suppression subtractive hybridization (SSH) provides high, representative coverage of regions that differ between simi...

rhizosphere soil samples were collected from olive orchards of golestan, guilan, zanjan and qom provinces. fluorescent pseudomonad strains were isolated from the collected samples on king’s b medium and their phenotypic features characterized as based on the standard bacteriological methods. for all the tested strains oxidase reaction, fluorescent pigment, growth at 4?c and arginine dihydrolase...

modern strains of broiler are highly susceptible to heart failure. heart related mortalities are observed predominantly in fast growing broiler chickens. the aim of this study was to investigate serotonin levels in ascitic and non ascitic broilers of three strains (ross, arbor acres plus and cobb). in this respect, a factorial test in crd method applied for the statistical analyses. in each of ...

avian infectious bronchitis is an acute and highly contagious disease that mainly causes respiratory symptoms in poultry. a number of serotypes and variants of the viral agent with poor cross-protection are the major problem to achieve desired immunity from vaccination. the s1 subunit of s glycoprotein (spike) is the major determinant of ibv so that a minor change in amino acid sequence of this...