When first secreted the radula consists of chitin and protein. In the bases and cusps of the teeth the reactions for tyrosine, tryptophane, and chitin become negative, and are replaced by reactions for amino groups and argentaffin material. The bases of the teeth become impregnated with iron. Subsequently the reactions for amino groups and argentaffin material become negative in the cusps and t...

Immunofluorescence and a new histochemical technique were employed to assay 226 breast cancer specimens for estrogen receptor. Results showed an overall correlation of 91 percent when compared to those of biochemical assays. The histochemical technique is rapid, easy to perform and reveals the same parameters as does immunofluorescence without the need for antiserum. Tumor cell receptor heterog...

The total dry mass of human erythrocytes was determined by both interference microscopy and x-ray microradiography. The determination of mass per unit area, and calculation of total dry mass per cell were simplified by changing the shape of the cells to spheres which were then flattened to discs of constant thickness when smeared on glass slides for measurement of fixed cells by interferometry,...

The beginning student of histology is frequently confused by the increasing burden of detail that is incorporated into the more encyclopedic texts. For such students the more condensed treatment available in this book will prove useful since it provides, in skeleton form, the essentials of the subject. In this sense the present (third) edition of this work fills a real need in providing the stu...

The presence of biogenic amines in cultured cells of mouse neuroblastoma C-1300 (clone NB-2a) was suggested by fluorescence-microscope histochemistry. Incubation in media containing L-[(14)C]tyrosine and L-[(14)C]tryptophan for 24 h, followed by high-voltage electrophoresis, radiochromatogram scanning, and scintillation counting, confirmed the presence of [(14)C]dopamine, [(14)C]norepinephrine,...

The presence of biogenic amines in cultured cells of mouse neuroblastoma C-1300 (clone NB-2a) was suggested by fluorescence-microscope histochemistry. Incubation in media containing L-[14C]tyrosine and L-[14C]tryptophan for 24 h, followed by high-voltage electrophoresis, radiochromatogram scanning, and scintillation counting, conf i rmed the presence of [~4C]dopamine, [~4C]norepinephrine, [~'C]...

We carried out this study with the purpose of analyzing the density of neurons of the myenteric plexus in the mesenteric, intermediate and antimesenteric regions of the ileum of rats. Whole-mounts stained with four different techniques were employed. Through countings under optic microscope in an area of 8.96 mm2 we found the following neuronal means with the techniques of Giemsa, NADH-diaphora...

Muscle biopsy is an important and essential tool in the diagnosis and treatment of muscle disease. For several decades, histological staining of paraffin-embedded tissue was used in muscle pathology. In the past few decades, freshfrozen enzyme histochemical techniques have become the standard for assessing muscle disease. They have been complemented by the use of electron microscopy to characte...

municable disease control to the newer responsibilities of the hazards of ionizing radiation and medical defense against atomic attack. The individual topics are adequately developed with emphasis, in the majority, on brevity of presentation rather than complete and exhaustive detail. References are listed after each chapter for the reader desiring more definitive information. "Epidemiologic Me...

M A T U R A T I O N and maintenance of ovarian function are under the control of the pituitary gonadotrophins. The capacity of the ovary to respond to FSH stimulation develops gradually with age, and is most pronounced when the animal approaches sexual maturity. In very young animals reactivity to gonadotrophins is found to be lacking, and up to a certain age, which is characteristic for each s...