نتایج جستجو برای: holo

تعداد نتایج: 1156  

2011
Yingjie Chen Shaofang Liu Yulin Cui Peng Jiang Huaxin Chen Fuchao Li Song Qin

The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized me...

Journal: :Angewandte Chemie 2014
Peng Zheng Yanyan Wang Hongbin Li

In metalloproteins, metal centers serve as active sites for a range of functional purposes and as important structural elements to facilitate protein folding and assembly. It is challenging to observe the reversible unfolding and refolding of metalloproteins because of a loss or decomposition of the metal center. Here, the reversible unfolding-refolding of the iron-sulfur protein rubredoxin was...

Journal: :Molecular and Cellular Biology 2014

Journal: :Experimental Hematology 2021

Red blood cell production, or erythropoiesis, is a proliferative process that requires tight regulation. Erythropoietin (Epo) glycoprotein cytokine plays major role in erythropoiesis by triggering erythroid progenitors/precursors of varying sensitivity. The concentration Epo bone marrow hypothesized to be suboptimal, and the survival cells has been suggested depend on However, key factors contr...

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