Matrix effects of different biological samples, including phosphate-buffered saline-bovine serum albumin (PBS-BSA), gelded horse serum, mouse serum, and mouse brain, were investigated for the determination of 17α- and β-estradiol using derivatization with dansyl chloride prior to LC-MS/MS. Matrix effects were evaluated based on the slopes of regression lines plotted from results obtained in bio...

1. Under the conditions stated pneumococci grow as readily in the serum of horses highly immunized to the homologous organism as they do in normal horse serum, and the rate of growth is not appreciably diminished. 2. This failure of immune serum to affect the growth rate is not altered when fresh rabbit blood, fresh human blood, or rabbit blister fluid is added in order to supply any hypothetic...

An antiserum specific for Vibrio cholerae exo-enterotoxin was produced by immunization of a horse with purified choleragenoid, a natural cholera toxoid. The serum has a high titer against the toxin antigen in passive hemagglutination tests and a respectable antipermeability factor activity. It also passively protected against choleragen-induced mouse foot edema. The serum was found to be useful...

1. The absorption of typhus sera (human or antityphus horse serum) with Proteus X-19 removes only the Proteus agglutinins, leaving the Rickettsia agglutinins intact. 2. The absorption of typhus sera with Mexican Rickettsiae removes the agglutinins for both the Rickettsia and Proteus X-19. 3. While normal or formalinized Rickettsiae are not agglutinated by anti-Proteus serum, these organisms-whe...

A method of detecting serum antinuclear factor by the use of antiglobulin conjugated with horseradish peroxidase has been compared with a standard procedure using a fluorescent label. The horseradish peroxidase conjugate was evaluated by block titration against positive serum and by its performance as a test reagent for screening and titrating sera. It is concluded that the horse-radish peroxid...

OBJECTIVES Published descriptions of the oral sugar test (OST) and insulin response test (IRT) have been inconsistent when specifying the protocol for fasting horses before testing. The purpose of our study was to examine the effect of fasting duration on blood glucose concentration, blood insulin concentration, glucose/insulin ratio, OST, and IRT results in horses. ANIMALS Ten healthy adult ...