This study aims to know the effect of water-extracted horseradish Moringa oleifera leaf and KW21 fertilizer combination application on density Nannochloropsis sp. It was conducted in Natural Food Laboratory State Polytechnique, Tual. The experiment used complete randomized design with 5 treatments 3 replications: A (25% extract + 75% KW21), B (50% 50% C (75% 25% D (positive control 100% E (nega...

The oxidation of phenol catalyzed by human myeloperoxidase and horseradish peroxidase resulted in extensive binding of phenol-derived metabolites to boiled rat liver protein. This binding paralleled closely the removal of phenol from the incubations and was inhibited from 83 to 99% by the addition of the antioxidants, ascorbate and glutathione, suggesting that metabolism and binding were occurr...

Systematic studies on phenol derivatives facilitates an explanation of the enhancement or inhibition of the luminol-H2O2-horseradish peroxidase system chemiluminescence. Factors that govern the enhancement are the one-electron reduction potentials of the phenoxy radicals (PhO./PhOH) vs. luminol radicals (L./LH-) and the reaction rates of the phenol derivatives with the compounds of horseradish ...

DNA methylation is a cell-type-specific epigenetic marker that essential for transcriptional regulation, silencing of repetitive and genomic imprinting. It also responsible the pathogenesis many diseases, including cancers. Herein, we present simple approach quantifying global in ovarian cancer patient plasma samples based on new class biopolymer nanobeads. Our utilises immune capture target el...

Clozapine is associated with a 0.8% incidence of agranulocytosis. Bioactivation to an unstable protein-reactive metabolite, identified as a nitrenium intermediate, has been implicated in the toxicity. In this study, we investigated whether the reactive metabolite is cytotoxic toward polymorphonuclear leukocytes and mononuclear leukocytes using horseradish peroxidase and H2O2 to generate the met...

Resonance Raman enhancement of derivatives and intermediates of horseradish peroxidase in the near ultraviolet (N-band excitation) results in intensity and enhancement patterns that are different from those normally observed within the porphyrin Soret (B-band) and alpha-beta (Q-band) absorptions. In particular it allows the resolution of resonance Raman spectra of horseradish peroxidase compoun...

The food antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are shown to be metabolized to covalent binding intermediates and various other metabolites by prostaglandin H synthase and horseradish peroxidase. BHA was extensively metabolized by horseradish peroxidase (80% conversion of parent BHA into metabolites) resulting in the formation of three dimeric products. O...

PURPOSE To determine whether acute hypertension (HT) is a risk factor for damage to the blood-brain barrier in carotid angiography with nonionic contrast media. METHODS Anesthetized rats received intravenous injections of technetium-99m-pertechnetate and horseradish peroxidase. Two groups of rats received metaraminol to raise their blood pressure to between 165 and 190 mmHg peak systolic and ...

Rat embryo fibroblasts cultured in the presence of monensin exhibited an inhibited uptake of horseradish peroxidase. The inhibition was detected after 3 h, after which time the cells became increasingly vacuolated; the concentration of monensin required to inhibit pinocytosis (0.4 microM for half-maximum inhibition at 18 h) was similar to that found by others to inhibit secretion. Both the exch...

Two limiting structures may be assigned to the primary derivative of peroxidases (Compound I) demanding either (a) total or (b) partial retention of peroxide components. It is now shown that (a) interaction of horseradish peroxidase with m-nitroperbenzoic acid, in 15 mM phosphate, pH 6.2, 25", leads to the stoichiometric formation of the enzymeperoxide derivative, m-nitrobenzoate and 1 proton; ...