Though many diagnostic PCR methods have been described, few techniques have been developed to rapidly and efficiently screen transformed bacterial colonies for plasmid presence (1). Unfortunately, PCR screening of a large number of colonies is time consuming and requires substantial amounts of polymerase and primers. In this report, we describe a two-step large-scale bacterial colony screening ...

Thermal fracture is prevalent in many engineering problems and one of the most devastating defects metal additive manufacturing. Due to interactive underlying physics involved, computational simulation such a process challenging. In this work, we propose thermo-mechanical phase-field model, which based on thermodynamically consistent derivation. The influence different coupling terms as damage-...

The detrimental precipitation at the 2205 duplex stainless steel (DSS) grain boundaries has characterized material to have poor workability after being hot-rolled. However, in microstructure is largely influenced by cooling rate or method. There an insufficient investigation of this steel's grade interfaces and rationale its workability. This study investigates influence heat treatment hot-roll...