A sensitive, fast, precise, reversed phasehigh performance liquid chromatography (RP-HPLC) method is developed for the determination of orphenadrine citrate in tablet dosage form. Linearity, precision, limit of detection (LOD), robustness and ruggedness, drug recovery and the system suitability parameters have been validated for the developed method. The HPLC conditions are methanol: acetonitri...

Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPARα), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a give...

A simple, rapid, precise and accurate HPLC – UV procedure has been developed to determine benzaldehyde, the toxic oxidation product of the widely used preservative and Co – solvent, benzyl alcohol, in injectable formulations. Benzaldehyde as a main potential impurity of benzyl alcohol may cause many toxic effects such as neurotoxicity, hypersensitivity in human and fatal toxic syndrome in prema...

The aim of current investigation was the validation of HPLC method with UV-detection for identification and determination of Yohimbine by using column Spherisorb ODS RP C18 (250 mm x 4.6 mm x 5 μm), temperature: 25 oC, mobile phase: methanol : acetonitrile : water = 70 : 20 : 10 v/v, flow rate: 2 ml/min., UV-detection: λ = 270 nm. HPLC method was validated in accordance of linearity, limit of d...

You, J., X. Zhao, Y. Suo, Y. Li, H. Wang, and G. Chen, Determination of LongChain Fatty Acids in Bryophyte Plant Extracts by HPLC with Fluorescence Detection and Identification with MS, J. Chromatogr. B 848:283–291, 2007. A sensitive method has been developed for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(ptoluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phena...

Analysis of pharmaceuticals and biomolecules using HPLC coupled to ICPMS and ESI-MS Andrew James Cartwright The work described within this thesis explores the use of HPLC coupled with ICPMS and ESI-MS in order to develop novel methods which overcome specific analytical challenges in the pharmaceutical industry. A membrane desolvation interface has been evaluated for coupling high performance li...

The optimum conditions of the mobile phase for HPLC with electrochemical detection (HPLC-ECD) were selected from among solvents of different grades with the standard deviation (SD) of area measurements based on FUMI (function of mutual information) theory as a criterion. In HPLC-ECD for determining baicalin and baicalein using a phosphoric acid-methanol-water mixture (0.5:60:40, v/v/v) as a mob...

The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections. Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins, benzofuran glycosides, flavonoids, and meroterpene were identified. The determination...

Molecular species of neutral phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were resolved by reversed-phase high-performance liquid chromatography (HPLC) using mobile phases of acetonitrile-methanol-water containing tetraalkylammonium phosphates (TAAPs). Competitive interactions of TAAPs and analyte solutes with a reversed-phase HPLC column resulted in reduced retent...

Reversed-phase high-performance liquid chromatographic (HPLC) behavior of fluorescent labeled phospholipids (PLs) was studied. Molecular species of phosphatidylethanolamine (PE) derivatized with fluorescein-, thiocarbamoyl-, pyrenesulfonyl-, and dimethylaminonaphthalenesulfonyl (dansyl)-fluorophores were separated on octadecylsilica with a mobile phase of acetonitrilemethanol-water in the prese...