A fast, specific, and sensitive method for determination of soy additives in processed meat products has been developed. The method is based on RP-HPLC analysis of the isoflavones daidzein and genistein using bisphenol A as an internal standard. This paper describes the development and optimisation of efficient extraction procedures (n-hexane/80% EtOH), concentration of isoflavones by solid-pha...

The influence of different brewing conditions on the concentration of the main caffeoylquinic acids (3-caffeoylquinic acid (3-CQA), 4-caffeoylquinic acid (4-CQA), and 5-caffeoylquinic acid (5-CQA)) was investigated. For this purpose, twenty-four coffee brews were extracted and analyzed using HPLC-DAD at 325 nm. Our findings demonstrate the great impact of brewing techniques on the caffeoylquini...

Kaempferol-3-O-gentiobioside, the major flavonoid glycoside in Indonesian Cassia alata was quantified in various parts of the plant. The mature leaf was found to contain the highest content of this metabolite. A decrease of the flavonoid content in the juvenile leaf during the period of October through December was also observed. The contents ranged from 2.0 to 5.0% and 1.0 to 4.0% in mature an...

background valproic acid is widely used as an anticonvulsant and mood-stabilizing drug in the treatment of epilepsy. determination of serum valproic acid is required in epilepsy therapy for efficient control of seizures. objectives in the present study, the gas chromatography method was applied to determine serum valproic acid and resulting data were compared with those obtained by high perform...

Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R=0.999), range (<2-100 microM), precision (inst...

A simple, precise, specific ,robust and rapid HPLC assay for the of measurement meloxicam in pharmaceutical formulations was developed and validated at Department of Pharmacy and Central Quality Assurance Laboratory, LCWU, Lahore. The solution of meloxicam standard was prepared in HPLC grade acetonitrile (5 μg.mL) and ultra sonicated for 15 minutes. One mL sample mixed well with one mL of HPLC ...

zataria multiflora boiss is used in traditional folk remedies as antiseptic, analgesic, carminative, anthelmintic medication. the main components of zataria multiflora are phenolic compounds such as thymol and carvacrol. the aim of this study was developed a simple and rapid method for determination of the thymol in different extracts of zataria multiflora by hplc method. the dried aerial parts...

Avocados are one of the important fruits in our diet, showing many health benefits. However, a significant amount avocados become defective as they transported throughout supply chain and refused by consumers, ending up at animal or pet feed manufacturers. Indeed, some previous evidence suggests that rejected still present high phenolic content can be reused drug pharmacological industry. There...

Traditional Chinese medicines (TCMs) are usually complex mixtures and contain hundreds of chemically different constituents, which make the quality control (QC) of crude drugs and their medical preparations extremely difficult. In the past years, with the rapid development of modern instrumental analysis and computer-aided data processing techniques, great progress has been made in the research...

determination of drug residues in food is of great importance due to their toxicity. long-term exposure with low level of drug residues could be important, especially in children. based on document study, oxytetracycline (otc) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...