The modification of high-performance liquid chromatography parameters leads to a more effective oligonucleotide-A purification process. Using various experimental such as buffer, concentration, and pH, method for optimizing the an on reverse-phase C18 column was created. To purify oligonucleotide-A, High-Performance Liquid Chromatography (HPLC), Ultraviolet-Visible Spectrophotometry (UV-Vis), C...

A method for the determination of phosphorylation sites in phosphoproteins based on column-switching high-performance liquid chromatography (HPLC) has been developed. The HPLC system consisted of a titania precolumn for the selective adsorption of phosphopeptides, an anion-exchange analytical column and a UV detector (215 nm). Rabbit muscle phosphorylase a (RPa) and porcine stomach pepsin (PSP)...

Hydrochlorothiazide (HCT) is a thiazide diuretic which reduces the resorption of electrolytes and consequently of water. Enalapril maleate (EM) is a derivative of 2 amino acids, L-alanine and L-proline, and is an antihypertensive and a vasodilator in congestive heart failure. The 2 drugs are used in association in the treatment of hypertension . Several methods are available in the literature f...

In the original publication, title was wrongly published. The correct should read as “Comparative study of UV-Chemometrics, Ratio spectra derivative and HPLC-QbD methods for simultaneous estimation carisoprodol, paracetamol caffeine in its triple combination formulation CARISOMA”.

There are various methods for the analysis of Potassium Sorbate and Sodium Benzoate in food products, but a rapid and reliable method for identification of these preservatives in Doogh (an Iranian traditional dairy drink) is a procedure, in which high performance liquid chromatography (HPLC) utilized and followed by UV diode array detection of the two preservatives. The aim of this case study w...

Abscisic acid (ABA) was purified from soybean (Glycine max [L.]) seed extract using a preparative high performance liquid chromatography (HPLC) procedure. The preparative procedure was rapid (70 minutes per sample), required no prior partitioning for purification and was quantitative as demonstrated with an internal standard of [2-(14)C]ABA, of which 98.9% was recovered.Following purification b...

Eight vegetables were pre-treated with and without sa­ ponification, and chromatographed by reversed-phase HPLC. Carote11Oid peaks were identified using seven reference standards. Samples extracts were also chromatographed llsing a mixed magnesia and diatomaceous earth column. Fractions were eluted using stepwise increases in the proportion of acetone in hexane, studied by UV-VIS ab­ sorption s...

A liquid chromatography (HPLC) method with UV detection was developed for determination of sodium hyaluronate in pharmaceutical formulation. Sodium hyaluronate is a polymer of disaccharides, composed of d-glucuronic acid and d-N-acetylglucosamine, linked via alternating β-1, 4 and β-1, 3 glycosidic bonds. Being a polymer compound it lacks a UV absorbing chromophore. In the absence of a UV absor...

Introduction . Coronavirus disease (COVID-19) is an acute infectious caused by SARS-CoV-2 (severe respiratory syndrome-related coronavirus 2). Favipiravir a synthetic prodrug with antiviral activity used for the treatment of COVID-19. There are oral and parenteral dosage forms favipiravir. Compared administration, administration has some advantages. Developing method determination favipiravir i...