Persistent HPV infection plays a major role in cervical cancer. This study was undertaken to identify HPV types in a cohort of Indian women with locally advanced cervical cancer as well as to determine the physical state and/or site of viral integration in the host genome. Pretreatment biopsies (n = 270) from patients were screened for HPV infection by a high throughput HPV genotyping assay bas...

Few studies have assessed the burden of human papillomavirus (HPV) infection in Pakistan. We aim to provide specific information on HPV-type distribution in invasive cervical cancer (ICC) in the country. A total of 280 formalin-fixed paraffin-embedded tissue blocks were consecutively selected from Shaukat Khanum Memorial Cancer Hospital and Research Centre (Lahore, Pakistan). HPV-DNA was detect...

Spheroids are generally self-assembled cells with the ability to generate their extracellular matrix, including complex cell-matrix and cell-cell interactions that resemble functional characteristics of corresponding tissue in vivo. The study aimed develop a three-dimensional (3D) spheroid system for cervical cancer cell lines, HeLa (HPV18), CaSki (HPV16), SiHa C33A (non-HPV), HT3 (non-HPV) as ...

The ability of host cells to activate apoptosis is perhaps the most potent weapon for helping eliminate viruses. Human papillomaviruses (HPV) several pathways, enabling infected avoid extrinsic and intrinsic pathways. incapacity prostatic epithelial induce leads invasive development prostate cancer. For pathogenesis cancer, risk factors have been reported; example, some viruses infectious disea...

The life cycle of human papillomaviruses (HPVs) is tightly linked to keratinocyte differentiation. Although expression of viral early genes is initiated immediately upon virus infection of undifferentiated basal cells, viral DNA amplification and late gene expression occur only in the mid to upper strata of the keratinocytes undergoing terminal differentiation. In this report, we show that the ...

BACKGROUND Infection with high-risk human papillomavirus (HPV) is the main cause of high-grade cervical intraepithelial neoplasia (CIN) and cancer. It has been suggested that information about high-risk HPV type-specific infection might make cervical cancer screening more effective. Persistent HPV infection could also be a useful screening marker. We estimated the long-term risk of high-grade C...