Introduction: Bone marrow (BM) is a known source of mesenchymal stem cells (MSCs) that are used for cell therapy. This study attempts to identify if the Wharton’s Jelly (WJ) is a suitable substitute for BM as a source for MSCs. Materials and Methods: A population of human WJ and BM stem cells were isolated and incubated with fluorescein conjugated antibodies for five specific MSC markers....

Weak microwave radiation (WMR) in our environment has raised health concerns in the public. Among those, communication frequencies are more than ever becoming widespread and their effects need thorough studies. A correct understanding of these effects in-vivo by in-vitro experiments shall preferentially use primary cells. In this study we compared non-modulated (CW) and modulated WMR exposure o...

brain injuries that caused by strokes (result of intra partum ischemia) are a frequent cause of prenatal mortality and morbidity with limited therapeutic options. transplanting human mesenchymal stem cells (hmscs) indicates improvement in hypoxic ischemic brain injury (hibd) by secretion growth factor stimulating repair processes (hmscs) known as multi potent cells which isolated from bone marr...

Spinal cord injury (SCI) is a neurologic disorder that have a significant impact on quality of life, life expectancy, and economic burden. SCI leads to irreversible neuronal loss and ultimately leads to paralysis. Mesenchymal stem cells (MSCs) are a promising source for cellular therapy because they have possessed the capacity of self-renewal and differentiation to several distinct mesenchymal ...

Human endometrium contains mesenchymal stem cells (eMSC) which have the ability to differentiate into three cell lineages and the potential in therapeutic applications. We hypothesize that using environmental induction in culture media such as dexamethasone, human recombinant insulin and human epidermal growth factor (hEGF) can differentiate endometrial stem cells into myoblast. These agents ha...

Strategies of Schwann cell (SC) transplantation to regenerate the peripheral nerve injury involves many limitations. Stem cells can be used as alternative cell sources for differentiation into SCs. Given the high potential of neural crest-derived stem cells for the generation of multiple cell lineages, in this research, we considered whether olfactory ecto-mesenchymal stem cells (OE-MSCs) derive...

Background: Mesenchymal stem cells (MSCs) are multipotent cells, capable of differentiating into different cell lines.They can sense their surrounding biochemical and biophysical factors, which play major roles in their differentiation toward different phenotypes. Therefore, the exposure of these cells to endothelial growth factor (VEGF) as well as hemodynamic biomechanical forces, which act on...

objective: zoledronic acid as a main treatment for osteoporosis has an important role in differentiation of mesenchymal stem cells. however, mechanism of osteoblastic differentiation induction by zoledronic acid is not well understood until now. in this research we evaluate zoledronic acid effect on methylation status of runx2 and dlx5 promoters. materials and methods: after isolation and expa...

in vitro expansion of mesenchymal stem cell (mscs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. on the other hand, some studies have indicated that bio (6-bromoindirubin-3-oxime) possesses mitogenic effects on cell culture. the objective of the present study was to examine the effect of bio on in vitro expansion and chondrogenic di...

introduction: recognition of human leukocyte antigens (hla) is of importance for hematopoietic stem cell transplantation. any hla-mismatches between the donor and recipient can cause graft rejection or other complications. in hla-typing experiments, usage of hla-known reference cells accompany with hla-unknown samples is obligatory. some international centers represent these cells with high exp...